ArticleScientific reports2019
Experimental validation of force inference in epithelia from cell to tissue scale.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Mechanics of compression-driven morphogenesis.Development (Cambridge, England) · 2026Review
- Harmonic fields and the mechanical response of a cellular monolayer to ablation.Journal of mathematical biology · 2026Article
- Cell rearrangement progression along the apical-basal axis is linked with 3D epithelial tissue structure.PRX life · 2026Article
- DynamicAtlas: a morphodynamic atlas for Drosophila development.Nature methods · 2026Article
- Mechanical constraints on cell plasticity: insights from the olfactory epithelium.Frontiers in cell and developmental biology · 2026Review
- A large-scale balloon model of confluent cells validates stress inference from geometry.npj biological physics and mechanics · 2026Article
- Nonmuscle myosin IIA (NMIIA) regulates anisotropic cell tension to maintain the hexagonal packing of mouse lens meridional row cells.Molecular biology of the cell · 2025Article
- Elimination of cells with local mismatch in differentiation timing contributes to synchronize tissue development.iScience · 2025Article
- Apical size reduction by macropinocytosis alleviates tissue crowding.Nature communications · 2025Article
- Force transmission is a master regulator of mechanical cell competition.Nature materials · 2025Article
- Origin of yield stress and mechanical plasticity in model biological tissues.Nature communications · 2025Article
- Tissue-like multicellular development triggered by mechanical compression in archaea.Science (New York, N.Y.) · 2025Article
- Measuring and manipulating mechanical forces during development.Nature cell biology · 2025Review
- A computational pipeline for spatial mechano-transcriptomics.Nature methods · 2025Article
- Phased ERK responsiveness and developmental robustness regulate teleost skin morphogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Apico-basal intercalations enable the integrity of curved epithelia.Computational and structural biotechnology journal · 2025Article
- Peeking into the future: inferring mechanics in dynamical tissues.Biochemical Society transactions · 2024Review
- Article
- Image-based force inference by biomechanical simulation.PLoS computational biology · 2024Article
- Single-cell morphometrics reveals T-box gene-dependent patterns of epithelial tension in the Second Heart field.Nature communications · 2024Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Morphogenesis relies on the active generation of forces, and the transmission of these forces to surrounding cells and tissues. Hence measuring forces directly in developing embryos is an essential task to study the mechanics of development. Among the experimental techniques that have emerged to measure forces in epithelial tissues, force inference is particularly appealing. Indeed it only requires a snapshot of the tissue, as it relies on the topology and geometry of cell contacts, assuming that forces are balanced at each vertex. However, establishing force inference as a reliable technique requires thorough validation in multiple conditions. Here we performed systematic comparisons of force inference with laser ablation experiments in four epithelial tissues from two animals, the fruit fly and the quail. We show that force inference accurately predicts single junction tension, tension patterns in stereotyped groups of cells, and tissue-scale stress patterns, in wild type and mutant conditions. We emphasize its ability to capture the distribution of forces at different scales from a single image, which gives it a critical advantage over perturbative techniques such as laser ablation. Overall, our results demonstrate that force inference is a reliable and efficient method to quantify the mechanical state of epithelia during morphogenesis, especially at larger scales when inferred tensions and pressures are binned into a coarse-grained stress tensor.
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