ArticleScientific reports2019
Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- An assay to quantify sexual commitment and stage conversion in the human malaria parasite Plasmodium falciparum.Nature protocols · 2026Review
- AP2-HS and a GDV1 regulatory feedback loop mediate environmental induction of sexual conversion in Plasmodium falciparum.Nature microbiology · 2026Article
- Elevated Plasmodium falciparum sexual conversion in HbAC and HbAS red blood cells in naturally infected malaria patients.PLoS pathogens · 2026Article
- Characterisation of the thermal and non-thermal stress conditions that activate the Plasmodium falciparum AP2-HS-dependent heat-shock response.PLoS pathogens · 2026Article
- Reversible host cell surface remodelling limits immune recognition and maximizes survival of Plasmodium falciparum gametocytes.PLoS pathogens · 2025Article
- Induction of gametocytogenesis in human malaria parasites: from stress to genome editing.Frontiers in microbiology · 2025Review
- Heterochromatin dynamics during the initial stages of sexual development in Plasmodium falciparum.Scientific reports · 2024Article
- Plasmodium falciparum gametocyte production correlates with genetic markers of parasite replication but is not influenced by experimental exposure to mosquito biting.EBioMedicine · 2024Article
- Household-level effects of seasonal malaria chemoprevention in the Gambia.Communications medicine · 2024Article
- Let it glow: genetically encoded fluorescent reporters in Plasmodium.Malaria journal · 2024Review
- Beyond the MEP Pathway: A novel kinase required for prenol utilization by malaria parasites.PLoS pathogens · 2024Article
- Transcriptomic approaches for identifying potential transmission blocking vaccine candidates in3 Biotech · 2023Review
- A versatile Plasmodium falciparum reporter line expressing NanoLuc enables highly sensitive multi-stage drug assays.Communications biology · 2023Article
- High-throughput analysis of the transcriptional patterns of sexual genes in malaria.Parasites & vectors · 2023Article
- Plasmodium falciparum Sexual Commitment Rate Variation among Clinical Isolates and Diverse Laboratory-Adapted Lines.Microbiology spectrum · 2022Article
- The protein aggregation inhibitor YAT2150 has potent antimalarial activity in Plasmodium falciparum in vitro cultures.BMC biology · 2022Article
- Efficient generation of mNeonGreenFrontiers in cellular and infection microbiology · 2022Article
- Revisiting the Effect of Pharmaceuticals on Transmission Stage Formation in the Malaria ParasiteFrontiers in cellular and infection microbiology · 2022Article
- CRISPR/Cas9-engineered inducible gametocyte producer lines as a valuable tool for Plasmodium falciparum malaria transmission research.Nature communications · 2021Article
- The private life of malaria parasites: Strategies for sexual reproduction.Molecular and biochemical parasitology · 2021Review
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transmission of malaria parasites from humans to mosquito vectors requires that some asexual parasites differentiate into sexual forms termed gametocytes. The balance between proliferation in the same host and conversion into transmission forms can be altered by the conditions of the environment. The ability to accurately measure the rate of sexual conversion under different conditions is essential for research addressing the mechanisms underlying sexual conversion, and to assess the impact of environmental factors. Here we describe new Plasmodium falciparum transgenic lines with genome-integrated constructs in which a fluorescent reporter is expressed under the control of the promoter of the gexp02 gene. Using these parasite lines, we developed a sexual conversion assay that shortens considerably the time needed for an accurate determination of sexual conversion rates, and dispenses the need to add chemicals to inhibit parasite replication. Furthermore, we demonstrate that gexp02 is expressed specifically in sexual parasites, with expression starting as early as the sexual ring stage, which makes it a candidate marker for circulating sexual rings in epidemiological studies.
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