ArticleNature communications2019
Detection of spacer precursors formed in vivo during primed CRISPR adaptation.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Bacteriophages inMicrobial genomics · 2025Article
- Harnessing CRISPR-Cas adaptation for RNA recording and beyond.BMB reports · 2024Review
- Identification of an anti-CRISPR protein that inhibits the CRISPR-Cas type I-B system inmSphere · 2023Article
- CRISPR-Cas adaptation in Escherichia coli.Bioscience reports · 2023Review
- Host nucleases generate prespacers for primed adaptation in theScience advances · 2022Article
- Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3.Nature communications · 2022Article
- Creating memories: molecular mechanisms of CRISPR adaptation.Trends in biochemical sciences · 2022Review
- Type III CRISPR-Cas Systems: Deciphering the Most Complex Prokaryotic Immune System.Biochemistry. Biokhimiia · 2021Review
- Article
- Prespacers formed during primed adaptation associate with the Cas1-Cas2 adaptation complex and the Cas3 interference nuclease-helicase.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Prophages are associated with extensive CRISPR-Cas auto-immunity.Nucleic acids research · 2020Article
- Adaptation induced by self-targeting in a type I-B CRISPR-Cas system.The Journal of biological chemistry · 2020Article
- Mapping CRISPR spaceromes reveals vast host-specific viromes of prokaryotes.Communications biology · 2020Article
- Processing and integration of functionally oriented prespacers in theThe Journal of biological chemistry · 2020Article
- Article
- Detection of CRISPR adaptation.Biochemical Society transactions · 2020Review
- Review
- Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Type I CRISPR-Cas loci provide prokaryotes with a nucleic-acid-based adaptive immunity against foreign DNA. Immunity involves adaptation, the integration of ~30-bp DNA fragments, termed prespacers, into the CRISPR array as spacers, and interference, the targeted degradation of DNA containing a protospacer. Interference-driven DNA degradation can be coupled with primed adaptation, in which spacers are acquired from DNA surrounding the targeted protospacer. Here we develop a method for strand-specific, high-throughput sequencing of DNA fragments, FragSeq, and apply this method to identify DNA fragments accumulated in Escherichia coli cells undergoing robust primed adaptation by a type I-E or type I-F CRISPR-Cas system. The detected fragments have sequences matching spacers acquired during primed adaptation and function as spacer precursors when introduced exogenously into cells by transformation. The identified prespacers contain a characteristic asymmetrical structure that we propose is a key determinant of integration into the CRISPR array in an orientation that confers immunity.
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