ArticleBMC genomics2019
Quantitative iTRAQ-based proteomic analysis of differentially expressed proteins in aging in human and monkey.
Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- Proteomic Markers of Aging and Longevity: A Systematic Review.International journal of molecular sciences · 2024Pooled it
- Current landscape of plasma proteomics from technical innovations to biological insights and biomarker discovery.Communications chemistry · 2025Article
- Paracrine Bone-Derived Senescent Secretome Induces Spatially Patterned ECM and Biomechanical Vulnerability in Human Brain Organoids.bioRxiv : the preprint server for biology · 2025Article
- Associations of age and sex with characteristics of extracellular vesicles and protein-enriched fractions of blood plasma.Aging cell · 2025Article
- Exploring the impact of age, sex and life experiences on plasma inflammatory profiles through comparative proteomics.Frontiers in immunology · 2025Article
- Evaluation of the efficacy of Tai Chi on the cognitive function of patients with mild cognitive dysfunction and research on its mechanism.Frontiers in aging neuroscience · 2025Article
- Model Organisms in Aging Research: Evolution of Database Annotation and Ortholog Discovery.Genes · 2024Article
- iTRAQ-Based Proteomic Profiling of Skin Aging Protective Effects ofMolecules (Basel, Switzerland) · 2024Article
- In platelet single donor apheresis, platelet factor 4 levels correlated with donor's age and decreased during storage.Scientific reports · 2024Article
- Identification of a hippocampal lncRNA-regulating network in a natural aging rat model.BMC neuroscience · 2022Article
- Multimerin-1 and cancer: a review.Bioscience reports · 2022Review
- The Aging Features of Thyrotoxicosis Mice: Malnutrition, Immunosenescence and Lipotoxicity.Frontiers in immunology · 2022Article
- Review
- Serum biomarker analysis at the protein level on pulmonary hypertension secondary to old anterior myocardial infarction.Pulmonary circulationArticle
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe underlying physiological mechanisms associated with aging are still complex and unclear. As a very important tissue of human body, the circulatory system also plays a very important role in the process of aging. In this study, we use the isobaric tags for relative and absolute quantification (iTRAQ) method to identify differentially expressed proteins in plasma for humans and monkeys between young and aged. Western blotting and behavioral experiment in mice were performed to validate the expression of the candidate protein.
resultsBetween the young / the old humans and the young / the old monkeys 74 and 69 proteins were found to be differently expressed, respectively. For the human samples, these included 38 up-regulated proteins and 36 down-regulated proteins (a fold change ≥1.3 or ≤ 0.667, p value ≤0.05).For the monkey samples, 51 up-regulated proteins and 18 down-regulated proteins (a fold change ≥1.3 or ≤ 0.667, p value ≤0.05). KEGG pathway analysis revealed that phagosome, focal adhesion, ECM-receptor interaction and PI3K/AKT signaling pathway were the most common pathways involved in aging. We found only IGFBP4 protein that existed in up-regulated proteins in aged both for human and monkey. In addition, the differential expression of IGFBP4 was validated by western blot analysis and IGFBP4 treatment mimicked aging-related cognitive dysfunction in mice.
conclusionsThis first, the integrated proteomics for the plasma protein of human and monkey reveal one protein-IGFBP4, which was validated by western blotting and behavioral analysis can promote the process of aging. And, iTRAQ analysis showed that proteolytic systems, and inflammatory responses plays an important role in the process of aging. These findings provide a basis for better understanding of the underlying mechanisms involved in aging.
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