ArticleFoods (Basel, Switzerland)2019
A Robust DNA Isolation Protocol from Filtered Commercial Olive Oil for PCR-Based Fingerprinting.
Article in Foods (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Quantitative and qualitative analysis of three DNA extraction methods from soybean, maize, and canola oils and investigation of the presence of genetically modified organisms (GMOs).Food chemistry. Molecular sciences · 2024Article
- Molecular Traceability Approach to Assess the Geographical Origin of Commercial Extra Virgin Olive Oil.Foods (Basel, Switzerland) · 2024Article
- Evaluation of genetic diversity among olive trees (Frontiers in plant science · 2024Article
- Review
- Microsatellite Markers in Olives (Foods (Basel, Switzerland) · 2021Review
- Molecular Approaches to Agri-Food Traceability and Authentication: An Updated Review.Foods (Basel, Switzerland) · 2021Review
- Bioactive Potential of Minor Italian Olive Genotypes from Apulia, Sardinia and Abruzzo.Foods (Basel, Switzerland) · 2021Article
- Article
- Procedures for DNA Extraction from Opium Poppy (Foods (Basel, Switzerland) · 2020Article
- Sustainable Palm Oil-The Role of Screening and Advanced Analytical Techniques for Geographical Traceability and Authenticity Verification.Molecules (Basel, Switzerland) · 2020Review
- Food Authentication: Techniques, Trends and Emerging Approaches.Foods (Basel, Switzerland) · 2020Article
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Authors and funding
12 authors.
Funding
Abstract
Extra virgin olive oil (EVOO) has elevated commercial value due to its health appeal, desirable characteristics and quantitatively limited production, and thus it has become an object of intentional adulteration. As EVOOs on the market might consist of a blend of olive varieties or sometimes even of a mixture of oils from different botanical species, an array of DNA-fingerprinting methods have been developed to check the varietal composition of the blend. Starting from a comparison between publicly available DNA extraction protocols, we set up a timely, low-cost, reproducible and effective DNA isolation protocol, which allows an adequate amount of DNA to be recovered even from commercial filtered EVOOs. Then, in order to verify the effectiveness of the DNA extraction protocol herein proposed, we applied PCR-based fingerprinting methods starting from the DNA extracted from three EVOO samples of unknown composition. In particular, genomic regions harboring nine simple sequence repeats (SSRs) and eight genotyping-by-sequencing-derived single nucleotide polymorphism (SNP) markers were amplified for authentication and traceability of the three EVOO samples. The whole investigation strategy herein described might favor producers in terms of higher revenues and consumers in terms of price transparency and food safety.
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Registered trials
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