ArticleJournal of neuroscience methods2020
Challenges of simultaneous measurements of brain extracellular GABA and glutamate in vivo using enzyme-coated microelectrode arrays.
Article in Journal of neuroscience methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Continuous monitoring of glutamate using electroactive templated polymers as synthetic molecular receptors.The Analyst · 2026Article
- Sampling Probes Microfabricated from Parylene‑C for In Vivo Neurochemical Monitoring.ACS measurement science au · 2025Article
- Age-related differences in resting glutamate levels and glutamate uptake in the hippocampus and frontal cortex of C57BL/6 mice.Neurobiology of aging · 2025Article
- A creatine efflux transporter in oligodendrocytes.The FEBS journal · 2025Article
- Recent Progress in Flexible Microelectrode Arrays for Combined Electrophysiological and Electrochemical Sensing.Biosensors · 2025Review
- Enhancing the sensitivity of gamma-aminobutyric acid and glutamate biosensors by electrochemically roughening platinum microelectrodes.Frontiers in neuroscience · 2025Article
- Design, Fabrication, and Implantation of Invasive Microelectrode Arrays as in vivo Brain Machine Interfaces: A Comprehensive Review.Journal of manufacturing processes · 2024Article
- Improving Sensitivity and Longevity of In Vivo Glutamate Sensors with Electrodeposited NanoPt.ACS applied materials & interfaces · 2024Article
- Editors' Choice-Review-The Future of Carbon-Based Neurochemical Sensing: A Critical Perspective.ECS sensors plus · 2023Review
- Microcontact printing of choline oxidase using a polycation-functionalized zwitterionic polymer as enzyme immobilization matrix.The Analyst · 2023Article
- Review
- GABACells · 2022Article
- Towards Multiplexed and Multimodal Biosensor Platforms in Real-Time Monitoring of Metabolic Disorders.Sensors (Basel, Switzerland) · 2022Review
- Brain-Implantable Multifunctional Probe for Simultaneous Detection of Glutamate and GABA Neurotransmitters: Optimization and In Vivo Studies.Micromachines · 2022Article
- Fabrication of Highly Sensitive Pt-black Electrochemical Sensors for GABA Detection.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2021Article
- Recent Advances in In Vivo Neurochemical Monitoring.Micromachines · 2021Review
- Novel microwire-based biosensor probe for simultaneous real-time measurement of glutamate and GABA dynamics in vitro and in vivo.Scientific reports · 2020Article
- Approaches to Monitor Circuit Disruption after Traumatic Brain Injury: Frontiers in Preclinical Research.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAlthough GABA is the major inhibitory neurotransmitter in the CNS, quantifying in vivo GABA levels has been challenging. The ability to co-monitor both GABA and the major excitatory neurotransmitter, glutamate, would be a powerful tool in both research and clinical settings. NEW
methodCeramic-based microelectrode arrays (MEAs) were used to quantify gamma-aminobutyric acid (GABA) by employing a dual-enzyme reaction scheme including GABase and glutamate oxidase (GluOx). Glutamate was simultaneously quantified on adjacent recording sites coated with GluOx alone. Endogenous glutamate was subtracted from the combined GABA and glutamate signal to yield a pure GABA concentration.
resultsElectrode sensitivity to GABA in conventional, stirred in vitro calibrations at pH 7.4 did not match the in vivo sensitivity due to diffusional losses. Non-stirred calibrations in agarose or stirred calibrations at pH 8.6 were used to match the in vivo GABA sensitivity. In vivo data collected in the rat brain demonstrated feasibility of the GABA/glutamate MEA including uptake of locally applied GABA, KCl-evoked GABA release and modulation of endogenous GABA with vigabatrin. COMPARISON WITH EXISTING
methodsImplantable enzyme-coated microelectrode arrays have better temporal and spatial resolution than existing off-line methods. However, interpretation of results can be complicated due to the multiple recording site and dual enzyme approach.
conclusionsThe initial in vitro and in vivo studies supported that the new MEA configuration may be a viable platform for combined GABA and glutamate measures in the CNS extending the previous reports to in vivo GABA detection. The challenges of this approach are emphasized.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.