ArticleReproductive toxicology (Elmsford, N.Y.)2020
Molecular characterization of a toxicological tipping point during human stem cell differentiation.
Article in Reproductive toxicology (Elmsford, N.Y.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The trial behind it
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Who cites it
13 citing papers in PubMed.
- Causality analysis of toxicological mechanisms in networked systems such as adverse outcome pathway networks.Archives of toxicology · 2026Review
- Article
- Community-Engaged Research and the Use of Open Access ToxVal/ToxRef In Vivo Databases and New Approach Methodologies (NAM) to Address Human Health Risks From Environmental Contaminants.Birth defects research · 2024Article
- Using high throughput screens to predict miscarriages with placental stem cells and long-term stress effects with embryonic stem cells.Birth defects research · 2022Review
- The DevTox Germ Layer Reporter Platform: An Assay Adaptation of the Human Pluripotent Stem Cell Test.Toxics · 2022Article
- Pluripotent stem cell assays: Modalities and applications for predictive developmental toxicity.Current research in toxicology · 2022Article
- Use of computational toxicology tools to predictCurrent research in toxicology · 2022Article
- Retinoic Acid Fluctuation Activates an Uneven, Direction-Dependent Network-Wide Robustness Response in Early Embryogenesis.Frontiers in cell and developmental biology · 2021Article
- Chlorpyrifos and ΔCurrent research in toxicology · 2021Review
- Retinoid signaling in skeletal development: Scoping the system for predictive toxicology.Reproductive toxicology (Elmsford, N.Y.) · 2021Review
- Using Live Imaging and FUCCI Embryonic Stem Cells to Rank DevTox Risks: Adverse Growth Effects of PFOA Compared With DEP Are 26 Times Faster, 1,000 Times More Sensitive, and 13 Times Greater in Magnitude.Frontiers in toxicology · 2021Article
- A p53-Dependent Checkpoint Induced upon DNA Damage Alters Cell Fate during hiPSC Differentiation.Stem cell reports · 2020Article
- Thalidomide Inhibits Human iPSC Mesendoderm Differentiation by Modulating CRBN-dependent Degradation of SALL4.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Endoderm gives rise to the gut tube in the early embryo. We differentiated human induced pluripotent stem cells (hiPSCs) to embryonic endoderm to identify a "tipping point" at which the developing system did not recover from perturbations caused by exposure to all-trans retinoic acid (ATRA). Differentiating hiPSC-derived endoderm exposed to five concentrations of ATRA between 0.001 and 10 μM at 6 h, 96 h, or 192 h was assessed for forkhead box A2 (FOXA2) protein expression and global gene transcript expression. A tipping point of 17 ± 11 nM was identified where patterns of differentially expressed genes supported a developmental trajectory shift indicating a potential teratogenic outcome. This concentration is between women's endogenous ATRA blood plasma levels and teratogenic levels of circulating isotretinoin, an ATRA isomer used to treat acne. Taken together, these data suggest that this approach is a sensitive method to identify a point of departure for chemicals that impact early embryonic development.
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