ArticlePloS one2019
The motility-based swim-up technique separates bull sperm based on differences in metabolic rates and tail length.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 67 citations in OpenAlex.
- Relationships Between Metabolism of Cryopreserved Equine Sperm Determined by the Seahorse Analyzer and Sperm Characteristics Measured by Flow Cytometry and Computer-Assisted Analysis of Motility.Veterinary sciences · 2025Article
- Time to revise: impact of methodology on boar sperm capacitation in vitro via phosphotyrosine patterns.BMC veterinary research · 2025Review
- Porcine milk small extracellular vesicles modulate peripheral blood mononuclear cell proteome in vitro.Scientific reports · 2025Article
- Frozen semen quality of Pasundan bulls with different individual variations.Open veterinary journal · 2025Article
- Bioenergetics of Rodent Spermatozoa.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Proteomic analysis of Toraya buffalo seminal plasma and sperm: uncovering insights to optimize reproductive success.Frontiers in veterinary science · 2025Article
- Effect of Urolithin A on Bovine Sperm Capacitation and In Vitro Fertilization.Animals : an open access journal from MDPI · 2024Article
- Age-dependent variations in proteomic characteristics of spermatozoa in Simmental bull.Frontiers in veterinary science · 2024Article
- Semen quality and frozen semen production in Pasundan bulls: A molecular weight perspective on seminal plasma and spermatozoa protein.Journal of advanced veterinary and animal research · 2023Article
- Strategies for Bacterial Eradication from Human and Animal Semen Samples: Current Options and Future Alternatives.Sensors (Basel, Switzerland) · 2023Review
- Comprehensive analysis of microRNA and metabolic profiles in bovine seminal plasma of different semen quality.Frontiers in veterinary science · 2023Article
- Effect of bovine viral diarrhea virus biotypes exposure on bovine gametes in early embryonic developmentVeterinary research forum : an international quarterly journal · 2023Article
- A proteomic approach to identifying spermatozoa proteins in Indonesian native Madura bulls.Frontiers in veterinary science · 2023Article
- Effect of High Viscosity on Energy Metabolism and Kinematics of Spermatozoa from Three Mouse Species Incubated under Capacitating Conditions.International journal of molecular sciences · 2022Article
- Opportunities and Limits of Conventional IVF versus ICSI: It Is Time to Come off the Fence.Journal of clinical medicine · 2022Review
- Relationships between Biomarkers of Oxidative Stress in Seminal Plasma and Sperm Motility in Bulls before and after Cryopreservation.Animals : an open access journal from MDPI · 2022Article
- Effect of a Novel Hydroxybenzoic Acid Based Mitochondria Directed Antioxidant Molecule on Bovine Sperm Function and Embryo Production.Animals : an open access journal from MDPI · 2022Article
- Bundle formation of sperm: Influence of environmental factors.Frontiers in endocrinology · 2022Article
- Mitoquinone does not improve sperm cryo-resistance in bulls.Reproduction in domestic animals = Zuchthygiene · 2022Article
- Capacitation promotes a shift in energy metabolism in murine sperm.Frontiers in cell and developmental biology · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Swim-up is a sperm purification method that is being used daily in andrology labs around the world as a simple step for in vitro sperm selection. This method accumulates the most motile sperm in the upper fraction and leaves sperm with low or no motility in the lower fraction. However, the underlying reasons are not fully understood. In this article, we compare metabolic rate, motility and sperm tail length of bovine sperm cells of the upper and lower fraction. The metabolic assay platform reveals oxygen consumption rates and extracellular acidification rates simultaneously and thereby delivers the metabolic rates in real time. Our study confirms that the upper fraction of bull sperm has not only improved motility compared to the cells in the lower fraction but also shows higher metabolic rates and longer flagella. This pattern was consistent across media of two different levels of viscosity. We conclude that the motility-based separation of the swim-up technique is also reflected in underlying metabolic differences. Metabolic assays could serve as additional or alternative, label-free method to evaluate sperm quality.
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