ArticlePLoS biology2019
Cell confinement reveals a branched-actin independent circuit for neutrophil polarity.
Article in PLoS biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Article
- Reconstitution of actomyosin networks in cell-sized liposomes dissects distinct mechanisms of membrane blebbing and symmetry breaking.Science advances · 2026Article
- Long-range mutual activation establishes Rho and Rac polarity during cell migration.Nature cell biology · 2026Article
- Article
- Chemical and Mechanical Regulation of Leukocyte Migration.Cold Spring Harbor perspectives in biology · 2026Review
- The Arp2/3 complex controls the development of homeostatic microglia.EMBO reports · 2026Article
- Spatially Distinct Myosin II Architectures Regulate Protrusion Dynamics and Directional Persistence during Immune Cell Migration.bioRxiv : the preprint server for biology · 2026Article
- Lysyl Oxidase Promotes Actin-Dependent Neutrophil Activation and Cytotoxicity Toward Retinal Endothelial Cells in Diabetes.Diabetes · 2026Article
- Article
- WAVE complex forms linear arrays at negative membrane curvature to instruct lamellipodia formation.The Journal of cell biology · 2025Article
- Confinement by Liquid-Liquid Interface Replicates In Vivo Neutrophil Deformations and Elicits Bleb-Based Migration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Reconstitution of actomyosin networks in cell-sized liposomes reveals distinct mechanical roles of cytoskeletal organization in membrane shape remodeling.bioRxiv : the preprint server for biology · 2025Article
- Model supports asymmetric regulation across the intercellular junction for collective cell polarization.PLoS computational biology · 2024Article
- Blebology: principles of bleb-based migration.Trends in cell biology · 2024Review
- Follow the flow: Actin and membrane act as an integrated system to globally coordinate cell shape and movement.Current opinion in cell biology · 2024Review
- Article
- Neutrophils actively swell to potentiate rapid migration.bioRxiv : the preprint server for biology · 2024Article
- Arp2/3 complex and the pentose phosphate pathway regulate late phases of neutrophil swarming.iScience · 2024Article
- Reduced Brain Cortex Angiogenesis in the Offspring of the Preeclampsia-Like Syndrome.Hypertension (Dallas, Tex. : 1979) · 2023Article
- Sensing their plasma membrane curvature allows migrating cells to circumvent obstacles.Nature communications · 2023Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Migratory cells use distinct motility modes to navigate different microenvironments, but it is unclear whether these modes rely on the same core set of polarity components. To investigate this, we disrupted actin-related protein 2/3 (Arp2/3) and the WASP-family verprolin homologous protein (WAVE) complex, which assemble branched actin networks that are essential for neutrophil polarity and motility in standard adherent conditions. Surprisingly, confinement rescues polarity and movement of neutrophils lacking these components, revealing a processive bleb-based protrusion program that is mechanistically distinct from the branched actin-based protrusion program but shares some of the same core components and underlying molecular logic. We further find that the restriction of protrusion growth to one site does not always respond to membrane tension directly, as previously thought, but may rely on closely linked properties such as local membrane curvature. Our work reveals a hidden circuit for neutrophil polarity and indicates that cells have distinct molecular mechanisms for polarization that dominate in different microenvironments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.