ArticleMolecular biology of the cell2019
Constitutive expression of a fluorescent protein reports the size of live human cells.
Article in Molecular biology of the cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Cell size-dependent mRNA transcription drives proteome remodeling.Cell reports · 2026Article
- Cell size modulates ferroptosis susceptibility.eLife · 2026Article
- Cell size modulates ferroptosis susceptibility.bioRxiv : the preprint server for biology · 2026Article
- The G1/S transition in mammalian stem cells in vivo is autonomously regulated by cell size.Nature communications · 2025Article
- MAPK and mTORC1 signaling converge to drive cyclin D1 protein production to enable cell cycle reentry in melanoma persister cells.Science signaling · 2025Article
- Oversized cells activate global proteasome-mediated protein degradation to maintain cell size homeostasis.eLife · 2025Article
- Genome dilution by cell growth drives starvation-like proteome remodeling in mammalian and yeast cells.Nature structural & molecular biology · 2024Article
- The GScience advances · 2024Article
- The G1/S transition is promoted by Rb degradation via the E3 ligase UBR5.bioRxiv : the preprint server for biology · 2024Article
- Article
- DAmFRET measures saturating concentrations and toxicities of protein phase transitions in vivo.Molecular biology of the cell · 2023Article
- Increasing cell size remodels the proteome and promotes senescence.Molecular cell · 2022Article
- Protein and lipid mass concentration measurement in tissues by stimulated Raman scattering microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Delineation of proteome changes driven by cell size and growth rate.Frontiers in cell and developmental biology · 2022Article
- The cell cycle inhibitor RB is diluted in G1 and contributes to controlling cell size in the mouse liver.Frontiers in cell and developmental biology · 2022Article
- Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification.Communications biology · 2021Article
- Computationally enhanced quantitative phase microscopy reveals autonomous oscillations in mammalian cell growth.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Cell growth dilutes the cell cycle inhibitor Rb to trigger cell division.Science (New York, N.Y.) · 2020Article
- On the Molecular Mechanisms Regulating Animal Cell Size Homeostasis.Trends in genetics : TIG · 2020Review
- A G1 Sizer Coordinates Growth and Division in the Mouse Epidermis.Current biology : CB · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Cell size is important for cell physiology because it sets the geometric scale of organelles and biosynthesis. A number of methods exist to measure different aspects of cell size, but each has significant drawbacks. Here, we present an alternative method to measure the size of single human cells using a nuclear localized fluorescent protein expressed from a constitutive promoter. We validate this method by comparing it to several established cell size measurement strategies, including flow cytometry optical scatter, total protein dyes, and quantitative phase microscopy. We directly compare our fluorescent protein measurement with the commonly used measurement of nuclear volume and show that our measurements are more robust and less dependent on image segmentation. We apply our method to examine how cell size impacts the cell division cycle and reaffirm that there is a negative correlation between size at cell birth and G1 duration. Importantly, combining our size reporter with fluorescent labeling of a different protein in a different color channel allows measurement of concentration dynamics using simple wide-field fluorescence imaging. Thus, we expect our method will be of use to researchers interested in how dynamically changing protein concentrations control cell fates.
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