ArticleQuantitative biology (Beijing, China)2019
High-throughput quantification of microbial birth and death dynamics using fluorescence microscopy.
Article in Quantitative biology (Beijing, China), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
- Differences in the fitness effects of traded resources shape traits and persistence in multi-mutualist communities.PloS one · 2026Article
- Collective production of hydrogen sulfide gas enables budding yeast lacking MET17 to overcome their metabolic defect.PLoS biology · 2023Article
- The ecology of wine fermentation: a model for the study of complex microbial ecosystems.Applied microbiology and biotechnology · 2021Review
- Article
- Metabolic excretion associated with nutrient-growth dysregulation promotes the rapid evolution of an overt metabolic defect.PLoS biology · 2020Article
- Metabolite-mediated modelling of microbial community dynamics captures emergent behaviour more effectively than species-species modelling.Journal of the Royal Society, Interface · 2019Article
- Disentangling strictly self-serving mutations from win-win mutations in a mutualistic microbial community.eLife · 2019Article
- Simulations reveal challenges to artificial community selection and possible strategies for success.PLoS biology · 2019Article
- Uncovering and resolving challenges of quantitative modeling in a simplified community of interacting cells.PLoS biology · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMicrobes live in dynamic environments where nutrient concentrations fluctuate. Quantifying fitness in terms of birth rate and death rate in a wide range of environments is critical for understanding microbial evolution and ecology.
methodsHere, using high-throughput time-lapse microscopy, we have quantified how
resultsFor lysine-requiring cells, very low concentrations of lysine are not detectably consumed and do not support cell birth, but delay the onset of death phase and reduce the death rate compared to no lysine. In contrast, in low hypoxanthine, hypoxanthine-requiring cells can produce new cells, yet also die faster than in the absence of hypoxanthine. For both strains, birth rates under various metabolite concentrations are better described by the sigmoidal-shaped Moser model than the well-known Monod model, while death rates can vary with metabolite concentration and time.
conclusionsOur work reveals how time-lapse microscopy can be used to discover non-intuitive microbial birth and death dynamics and to quantify growth rates in many environments.
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