ArticleChemCatChem2019
Engineering a Seven Enzyme Biotransformation using Mathematical Modelling and Characterized Enzyme Parts.
Article in ChemCatChem, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Biocatalytic Carboxylic Acid Reduction and Transamination in Cell-Free Lysates at High Substrate Loading.ChemSusChem · 2026Article
- Unique kinetic behavior of a secondary alcohol dehydrogenase: from a reaction engineering point of view.Bioprocess and biosystems engineering · 2026Article
- Analysis of variable-order fractional enzyme kinetics model with time delay.Scientific reports · 2025Article
- Optimization of Biocatalytic Rhododendrol Production from Biogenic Rhododendrol Glycosides.ACS sustainable chemistry & engineering · 2024Article
- Enhanced ε-Poly-L-Lysine Production inBiomolecules · 2024Article
- Multi-enzyme catalysed processes using purified and whole-cell biocatalysts towards a 1,3,4-substituted tetrahydroisoquinoline.RSC advances · 2023Article
- Characterization of Carboxylic Acid Reductase fromPolymers · 2022Article
- One-Step Biocatalytic Synthesis of Sustainable Surfactants by Selective Amide Bond Formation.Angewandte Chemie (International ed. in English) · 2022Article
- Complexity reduction and opportunities in the design, integration and intensification of biocatalytic processes for metabolite synthesis.Frontiers in bioengineering and biotechnology · 2022Review
- Production of the Carboxylate Reductase from Nocardia otitidiscaviarum in a Soluble, Active Form for in vitro Applications.Chembiochem : a European journal of chemical biology · 2021Article
- RetroBioCat as a computer-aided synthesis planning tool for biocatalytic reactions and cascades.Nature catalysis · 2021Article
- Preparation of a Copper Polyphosphate Kinase Hybrid Nanoflower and Its Application in ADP Regeneration from AMP.ACS omega · 2020Article
- Engineering a Seven Enzyme Biotransformation using Mathematical Modelling and Characterized Enzyme Parts.ChemCatChem · 2019Article
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Authors and funding
6 authors.
Funding
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Abstract
Multi-step enzyme reactions offer considerable cost and productivity benefits. Process models offer a route to understanding the complexity of these reactions, and allow for their optimization. Despite the increasing prevalence of multi-step biotransformations, there are few examples of process models for enzyme reactions. From a toolbox of characterized enzyme parts, we demonstrate the construction of a process model for a seven enzyme, three step biotransformation using isolated enzymes. Enzymes for cofactor regeneration were employed to make this in vitro reaction economical. Good modelling practice was critical in evaluating the impact of approximations and experimental error. We show that the use and validation of process models was instrumental in realizing and removing process bottlenecks, identifying divergent behavior, and for the optimization of the entire reaction using a genetic algorithm. We validated the optimized reaction to demonstrate that complex multi-step reactions with cofactor recycling involving at least seven enzymes can be reliably modelled and optimized.
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