Evidence map›Paper›PMID 31597962›Full record

ArticleNature2019

Decoding human fetal liver haematopoiesis.

Dorin-Mirel Popescu, Rachel A Botting, Emily Stephenson, Kile Green, Simone Webb, Laura Jardine, Emily F Calderbank, Krzysztof Polanski, Issac Goh, Mirjana Efremova and 46 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 385 papers.

0numbers the graph read from it
0cells of the map it votes in
385citing papers in PubMed
28.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

385 citing papers in PubMed, 622 citations in OpenAlex.

  1. Trial
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  9. Prenatal PMNature communications · 2026
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  12. The endothelial scavenger receptorbioRxiv : the preprint server for biology · 2026
    Article
  13. Article
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  15. Review
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  17. Article
  18. Article
  19. Article
  20. Article

325 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

56 authors at 11 institutions in 3 countries.

Dorin-Mirel Popescu *Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Rachel A Botting *Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Emily Stephenson *Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Kile GreenInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Simone WebbInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Laura JardineInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Emily F CalderbankDepartment of Haematology and Wellcome and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.
Krzysztof PolanskiWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Issac GohInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Mirjana EfremovaWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Meghan AcresInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Daniel MaunderInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Peter VeghInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Yorick GittonSorbonne Université, INSERM, CNRS, Institut de la Vision, Paris, France.
Jong-Eun ParkWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Roser Vento-TormoWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Zhichao MiaoWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
David DixonInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Rachel RowellInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
David McDonaldInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
James FletcherInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Elizabeth PoynerInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Gary ReynoldsInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Michael MatherInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Corina MoldovanDepartment of Pathology, Newcastle Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK.
Lira MamanovaWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Frankie GreigInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Matthew D YoungWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Kerstin B MeyerWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Steven LisgoInstitute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, UK.
Jaume BacarditSchool of Computing, Newcastle University, Newcastle upon Tyne, UK.
Andrew FullerInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Ben MillarInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Barbara InnesInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Susan LindsayInstitute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, UK.
Michael J T StubbingtonWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Monika S KowalczykKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Bo LiKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Orr AshenbergKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Marcin TabakaKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Danielle DionneKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Timothy L TickleKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Michal SlyperKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Orit Rozenblatt-RosenKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Andrew FilbyInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Peter CareyBroad Institute of Harvard and MIT, Cambridge, MA, USA.
Alexandra-Chloé VillaniCenter for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, MA, USA.
Anindita RoyDepartment of Paediatrics, University of Oxford, Oxford, UK.
Aviv RegevKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Alain ChédotalSorbonne Université, INSERM, CNRS, Institut de la Vision, Paris, France.
Irene RobertsDepartment of Paediatrics, University of Oxford, Oxford, UK.
Berthold GöttgensDepartment of Haematology and Wellcome and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.
Sam BehjatiWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK. sb31@sanger.ac.uk.
Elisa LaurentiDepartment of Haematology and Wellcome and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. el422@cam.ac.uk.
Sarah A TeichmannWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK. st9@sanger.ac.uk.
Muzlifah HaniffaInstitute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK. m.a.haniffa@newcastle.ac.uk.
Newcastle University · GBBroad Institute · USWellcome Sanger Institute · GBUniversity of Cambridge · GBNIHR Newcastle Biomedical Research Centre · GBUniversity of Oxford · GBInserm · FRInstitut de la Vision · FRMassachusetts General Hospital · USNewcastle upon Tyne Hospitals NHS Foundation Trust · GBWellcome/MRC Cambridge Stem Cell Institute · GB

Funding

Medical Research Council MC_PC_12009Medical Research Council MC_PC_16040Medical Research Council MC_UU_00016/13Medical Research Council MC_UU_12009/14Medical Research Council MR/R006237/1Wellcome TrustWellcome Trust 107931Wellcome Trust 110104Wellcome Trust 206194Wellcome Trust 211276
6 · The paper itself

Abstract

Definitive haematopoiesis in the fetal liver supports self-renewal and differentiation of haematopoietic stem cells and multipotent progenitors (HSC/MPPs) but remains poorly defined in humans. Here, using single-cell transcriptome profiling of approximately 140,000 liver and 74,000 skin, kidney and yolk sac cells, we identify the repertoire of human blood and immune cells during development. We infer differentiation trajectories from HSC/MPPs and evaluate the influence of the tissue microenvironment on blood and immune cell development. We reveal physiological erythropoiesis in fetal skin and the presence of mast cells, natural killer and innate lymphoid cell precursors in the yolk sac. We demonstrate a shift in the haemopoietic composition of fetal liver during gestation away from being predominantly erythroid, accompanied by a parallel change in differentiation potential of HSC/MPPs, which we functionally validate. Our integrated map of fetal liver haematopoiesis provides a blueprint for the study of paediatric blood and immune disorders, and a reference for harnessing the therapeutic potential of HSC/MPPs.

Indexed as

HematopoiesisBlood CellsCellular MicroenvironmentFemaleFetusFlow CytometryGene Expression ProfilingHumansLiverLymphoid TissueSingle-Cell AnalysisStem Cells

Identifiers

PMID31597962
PMCPMC6861135
OpenAlexW2980292232

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.