ArticleParasites & vectors2019
Quantitative proteomic analysis and functional characterization of Acanthamoeba castellanii exosome-like vesicles.
Article in Parasites & vectors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 50 citations in OpenAlex.
- The Therapeutic Potential of Exosomes in Ocular Surface Diseases.Biomolecules · 2026Review
- Exosomes in corneal diseases: advances in diagnosis and therapy.Frontiers in cell and developmental biology · 2026Review
- Proteomic identification of Acanthamoeba castellanii excretory-secretory proteins associated with Klebsiella pneumoniae capsule enlargement: a candidate role for C-type lysozyme.Parasite (Paris, France) · 2026Article
- Small excretory-secretory proteins of Acanthamoeba castellanii genotype T4 upregulate the expression of capsule-associated genes via the Rcs two-component system in Klebsiella pneumoniae.Parasites & vectors · 2025Article
- Trichomonas vaginalis extracellular vesicles activate the NLRP3 inflammasome and TLR3-mediated inflammatory cascades in host cells.PLoS pathogens · 2025Article
- Characterization ofFrontiers in cellular and infection microbiology · 2025Article
- Detection of immunogenic protein components in excretion/secretion products of Acanthamoeba T5 using polyclonal antibodies.Memorias do Instituto Oswaldo Cruz · 2025Article
- Chloroquine induces eryptosis inFrontiers in cellular and infection microbiology · 2025Article
- Comprehensive characterization of extracellular vesicles produced by environmental (Neff) and clinical (T4) strains ofmSystems · 2024Article
- Overview of extracellular vesicles in pathogens with special focus on human extracellular protozoan parasites.Memorias do Instituto Oswaldo Cruz · 2024Review
- Providing anFrontiers in microbiology · 2024Article
- Extracellular Vesicles Secreted byMicroorganisms · 2023Article
- Article
- Guidelines for the purification and characterization of extracellular vesicles of parasites.Journal of extracellular biology · 2023Article
- Article
- Enhancement of capsular hypermucoviscosity in Klebsiella pneumoniae by Acanthamoeba.PLoS neglected tropical diseases · 2023Article
- Quantitative proteomic analysis of extracellular vesicles in response to baculovirus infection of a Trichoplusia ni cell line.PloS one · 2023Article
- Characterization of the extracellular vesicles, ultrastructural morphology, and intercellular interactions of multiple clinical isolates of the brain-eating amoeba,Frontiers in microbiology · 2023Article
- Eimeria falciformis secretes extracellular vesicles to modulate proinflammatory response during interaction with mouse intestinal epithelial cells.Parasites & vectors · 2022Article
- Candida albicans Hyphal Extracellular Vesicles Are Different from Yeast Ones, Carrying an Active Proteasome Complex and Showing a Different Role in Host Immune Response.Microbiology spectrum · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundPathogenic protozoans use extracellular vesicles (EVs) for intercellular communication and host manipulation. Acanthamoeba castellanii is a free-living protozoan that may cause severe keratitis and fatal granulomatous encephalitis. Although several secreted molecules have been shown to play crucial roles in the pathogenesis of Acanthamoeba, the functions and components of parasite-derived EVs are far from understood.
methodsPurified EVs from A. castellanii were confirmed by electron microscopy and nanoparticle tracking analysis. The functional roles of parasite-derived EVs in the cytotoxicity to and immune response of host cells were examined. The protein composition in EVs from A. castellanii was identified and quantified by LC-MS/MS analysis.
resultsEVs from A. castellanii fused with rat glioma C6 cells. The parasite-derived EVs induced an immune response from human THP-1 cells and a cytotoxic effect in C6 cells. Quantitative proteomic analysis identified a total of 130 proteins in EVs. Among the identified proteins, hydrolases (50.2%) and oxidoreductases (31.7%) were the largest protein families in EVs. Furthermore, aminopeptidase activities were confirmed in EVs from A. castellanii.
conclusionsThe proteomic profiling and functional characterization of EVs from A. castellanii provide an in-depth understanding of the molecules packaged into EVs and their potential mechanisms mediating the pathogenesis of this parasite.
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