Evidence map›Paper›PMID 31597013›Full record

ArticleTransboundary and emerging diseases2020

Development of a multiplex RT-PCR for the detection of major diarrhoeal viruses in pig herds in China.

Guangming Ding, Yuguang Fu, Baoyu Li, Jianing Chen, Jianlin Wang, Baishuang Yin, Wanli Sha, Guangliang Liu

Open access · bronzeAbstract read
In one paragraph

Article in Transboundary and emerging diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.

0numbers the graph read from it
0cells of the map it votes in
63citing papers in PubMed
8.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

63 citing papers in PubMed, 108 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. CRISPR/Cas12a-Based One-Tube RT-RAA Assay for PoRV Genotyping.International journal of molecular sciences · 2025
    Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article

3 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Guangming DingState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Yuguang FuState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Baoyu LiState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Jianing ChenState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Jianlin WangSchool of Life Sciences, Lanzhou University, Lanzhou, China.
Baishuang YinCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China.
Wanli ShaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China.
Guangliang LiuState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.ORCID https://orcid.org/0000-0001-8158-5749
Lanzhou Veterinary Research Institute · CNJilin Agricultural Science and Technology University · CNLanzhou University · CN

Funding

Elite Youth Program of CAAS, and partly by China Central Public-interest Scientific Institution Basal Research Fund 1610312018002National Key R&D Program of China 2016YFD0500103National Natural Science Foundation of China 31572498National Natural Science Foundation of China 31702209National Natural Science Foundation of China 31972689
6 · The paper itself

Abstract

The major enteric RNA viruses in pigs include porcine epidemic diarrhoea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine rotavirus A (PRV-A), porcine kobuvirus (PKV), porcine sapovirus (PSaV) and porcine deltacoronavirus (PDCoV). For differential diagnosis, a multiplex RT-PCR method was established on the basis of the N genes of TGEV, PEDV and PDCoV, the VP7 gene of PRV-A, and the polyprotein genes of PKV and PSaV. This multiplex RT-PCR could specifically detect TGEV, PEDV, PDCoV, PRV-A, PKV and PSaV without cross-reaction to any other major viruses circulating in Chinese pig farms. The limit of detection of this method was as low as 10

Indexed as

AnimalsChinaCoronavirus InfectionsDiagnosis, DifferentialDiarrheaFecesGastroenteritis, Transmissible, of SwineKobuvirusMultiplex Polymerase Chain ReactionPorcine epidemic diarrhea virusReverse Transcriptase Polymerase Chain ReactionRotavirusSensitivity and SpecificitySwineSwine Diseasesdiarrhoeamultiplex RT-PCRswine enteric viruses

Identifiers

PMID31597013
PMCPMC7168528
OpenAlexW2979410757

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.