ArticlePLoS biology2019
Single-cell RNA sequencing reveals regulatory mechanism for trophoblast cell-fate divergence in human peri-implantation conceptuses.
Article in PLoS biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 76 citations in OpenAlex.
- Combined EP300 and CREBBP Expression Levels Regulate Human Trophoblast Differentiation.International journal of molecular sciences · 2026Article
- ANGPTL4 in gestational diabetes: a diagnostic biomarker linked to placental senescence.Functional & integrative genomics · 2026Article
- A longitudinal single-nucleus transcriptomic atlas of bovine placentation reveals dynamic cellular hierarchies and regulatory programs.Genome biology · 2026Article
- Free apical surface hetero-cellular CDH1 homophilic binding is a major mediator of blastocyst-endometrium interaction in humans.Human reproduction (Oxford, England) · 2026Article
- Trophoblast Fusion Deficiency in Placenta-Related Pregnancy Disorders.Advances in experimental medicine and biology · 2026Review
- Trophoblast invasion biology: from normal implantation to accreta spectrum and choriocarcinoma.Vascular biology (Bristol, England) · 2026Review
- Activity of the SWI/SNF complex is indispensable for syncytiotrophoblast formation.Development (Cambridge, England) · 2025Article
- Integrative multiomic and immune profiling of lung adenocarcinoma: molecular landscapes, gene expression, and treatment response insights.The oncologist · 2025Article
- Two classes of syncytiotrophoblast nuclei form following BAP-driven human pluripotent stem cell differentiation.Placenta · 2025Article
- Single Cell RNA Sequencing and Its Impact on Understanding Human Embryo Development.International journal of molecular sciences · 2025Review
- Review
- The impact of dynamic caudal type homeobox 2 expression on the differentiation of human trophoblast lineage during implantation.Cell proliferation · 2024Article
- In vitro culture of cynomolgus monkey embryos from blastocyst to early organogenesis.Nature protocols · 2024Review
- Distinct pathways drive anterior hypoblast specification in the implanting human embryo.Nature cell biology · 2024Article
- Dynamic and distinct histone modifications facilitate human trophoblast lineage differentiation.Scientific reports · 2024Article
- MICA: a multi-omics method to predict gene regulatory networks in early human embryos.Life science alliance · 2024Article
- Decoding the molecular pathways governing trophoblast migration and placental development; a literature review.Frontiers in endocrinology · 2024Review
- Exploring myometrial microenvironment changes at the single-cell level from nonpregnant to term pregnant states.Physiological genomics · 2024Article
- Enzymatic Digestion and Single Cell Isolation of Peri-implantation Stage Human Trophoblast Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Signaling mechanisms that direct cell fate specification and morphogenesis in human embryonic stem cells-based models of human gastrulation.Emerging topics in life sciences · 2023Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multipotent trophoblasts undergo dynamic morphological movement and cellular differentiation after conceptus implantation to generate placenta. However, the mechanism controlling trophoblast development and differentiation during peri-implantation development in human remains elusive. In this study, we modeled human conceptus peri-implantation development from blastocyst to early postimplantation stages by using an in vitro coculture system and profiled the transcriptome of 476 individual trophoblast cells from these conceptuses. We revealed the genetic networks regulating peri-implantation trophoblast development. While determining when trophoblast differentiation happens, our bioinformatic analysis identified T-box transcription factor 3 (TBX3) as a key regulator for the differentiation of cytotrophoblast (CT) into syncytiotrophoblast (ST). The function of TBX3 in trophoblast differentiation is then validated by a loss-of-function experiment. In conclusion, our results provided a valuable resource to study the regulation of trophoblasts development and differentiation during human peri-implantation development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.