ArticleGenome biology and evolution2019
Genome Assembly of the A-Group Wolbachia in Nasonia oneida Using Linked-Reads Technology.
Article in Genome biology and evolution, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Genome assembly and annotation of the parasitoid jewel wasp Nasonia oneida.Scientific data · 2025Article
- Review
- New insights into the genome and transmission of the microsporidian pathogenFrontiers in microbiology · 2023Article
- Article
- InnovativeFrontiers in genetics · 2022Review
- Phylogenomic Analysis of Wolbachia Strains Reveals Patterns of Genome Evolution and Recombination.Genome biology and evolution · 2020Article
- Genome Report: Whole Genome Sequence and Annotation of the Parasitoid Jewel WaspG3 (Bethesda, Md.) · 2020Article
- Bioinformatics and Computational Tools for Next-Generation Sequencing Analysis in Clinical Genetics.Journal of clinical medicine · 2020Review
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wolbachia are obligate intracellular bacteria which commonly infect various nematode and arthropod species. Genome sequences have been generated from arthropod samples following enrichment for the intracellular bacteria, and genomes have also been assembled from arthropod whole-genome sequencing projects. However, these methods remain challenging for infections that occur at low titers in hosts. Here we report the first Wolbachia genome assembled from host sequences using 10× Genomics linked-reads technology. The high read depth attainable by this method allows for recovery of intracellular bacteria that are at low concentrations. Based on the depth differences (714× for the insect and 59× for the bacterium), we assembled the genome of a Wolbachia in the parasitoid jewel wasp species Nasonia oneida. The final draft assembly consists of 1,293, 06 bp in 47 scaffolds with 1,114 coding genes and 97.01% genome completeness assessed by checkM. Comparisons of the five Multi Locus Sequence Typing genes revealed that the sequenced Wolbachia genome is the A1 strain (henceforth wOneA1) previously reported in N. oneida. Pyrosequencing confirms that the wasp strain lacks A2 and B types previously detected in this insect, which were likely lost during laboratory culturing. Assembling bacterial genomes from host genome projects can provide an effective method for sequencing bacterial genomes, even when the infections occur at low density in sampled tissues.
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