ArticleJournal of Zhejiang University. Science. B
Development and optimization of an intergeneric conjugation system and analysis of promoter activity in Streptomyces rimosus M527.
Article in Journal of Zhejiang University. Science. B. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
- LipReg4 deletion blocks hypertension induced by gut S. aureofaciens Tü117.Hypertension research : official journal of the Japanese Society of Hypertension · 2026Article
- Construction of a library of gene expression elements in industrialSynthetic and systems biotechnology · 2026Article
- Fluorescence-Based Soil Survival Analysis of the Xenobiotic- and Metal-DetoxifyingInternational journal of molecular sciences · 2025Article
- Enhancing conjugation from E. coli to Streptomyces coelicolor by incorporating traJ into mobilizable plasmids.Applied microbiology and biotechnology · 2025Article
- Study on the regulatory mechanism of NsdAMicrobial cell factories · 2025Article
- Enhancing rufomycin production by CRISPR/Cas9-based genome editing and promoter engineering inSynthetic and systems biotechnology · 2025Article
- Transposon-based identification of genes involved in the rimocidin biosynthesis in Streptomyces rimosus M527.World journal of microbiology & biotechnology · 2023Article
- Identification of RimR2 as a positive pathway-specific regulator of rimocidin biosynthesis in Streptomyces rimosus M527.Microbial cell factories · 2023Article
- Improvement of Rimocidin Biosynthesis by Increasing Supply of Precursor Malonyl-CoA via Over-expression of Acetyl-CoA Carboxylase in Streptomyces rimosus M527.Current microbiology · 2022Article
- Enabling Efficient Genetic Manipulations in a Rare ActinomyceteFrontiers in microbiology · 2022Article
- Development of aJournal of Zhejiang University. Science. B · 2021Article
- Identification of a gene from Streptomyces rimosus M527 negatively affecting rimocidin biosynthesis and morphological differentiation.Applied microbiology and biotechnology · 2020Article
- Porcine circovirus 3 capsid protein induces autophagy in HEK293T cells by inhibiting phosphorylation of the mammalian target of rapamycin.Journal of Zhejiang University. Science. B · 2020Article
- Coordinated regulation for nature products discovery and overproduction inSynthetic and systems biotechnology · 2020Article
- Effects ofJournal of Zhejiang University. Science. BArticle
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6 authors.
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Abstract
An efficient genetic transformation system and suitable promoters are essential prerequisites for gene expression studies and genetic engineering in streptomycetes. In this study, firstly, a genetic transformation system based on intergeneric conjugation was developed in Streptomyces rimosus M527, a bacterial strain which exhibits strong antagonistic activity against a broad range of plant-pathogenic fungi. Some experimental parameters involved in this procedure were optimized, including the conjugative media, ratio of donor to recipient, heat shock temperature, and incubation time of mixed culture. Under the optimal conditions, a maximal conjugation frequency of 3.05×10
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