Evidence map›Paper›PMID 31595552›Full record

ArticleClinical anatomy (New York, N.Y.)2020

Age related changes seen in human cornea in formalin fixed sections and on biomicroscopy in living subjects: A comparison.

Samanta Taurone, Selenia Miglietta, Marialuisa Spoletini, Janos Feher, Marco Artico, Veronica Papa, Roberto Matassa, Giuseppe Familiari, Pietro Gobbi, Alessandra Micera

Open access · hybridAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical anatomy (New York, N.Y.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 27 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Samanta TauroneIRCCS-Fondazione Bietti, Rome, Italy.ORCID https://orcid.org/0000-0002-0354-894X
Selenia MigliettaDepartment of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, "Sapienza" University of Rome, Rome, Italy.
Marialuisa SpoletiniDepartment of Sensory Organs, "Sapienza" University of Rome, Rome, Italy.
Janos FeherDivision of Ophthalmology, University of Pecs, Pecs, Hungary.
Marco ArticoDepartment of Sensory Organs, "Sapienza" University of Rome, Rome, Italy.
Veronica PapaDepartment of Motor Sciences and Wellness, University of Naples "Partenope", Naples, Italy.
Roberto MatassaDepartment of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, "Sapienza" University of Rome, Rome, Italy.
Giuseppe FamiliariDepartment of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, "Sapienza" University of Rome, Rome, Italy.
Pietro GobbiDepartment of Biomolecular Sciences, University of Urbino "Carlo Bo", Urbino, Italy.
Alessandra MiceraIRCCS-Fondazione Bietti, Rome, Italy.
Sapienza University of Rome · ITFondazione G.B. Bietti · ITParthenope University of Naples · ITUniversity of Pecs · HUUniversity of Urbino · IT

Funding

Fondazione RomaMinistry of Health
6 · The paper itself

Abstract

The purpose of our experimental research was to assess the effects of aging on the main corneal structures in healthy corneas. Small, human cornea samples were collected from 20 Caucasian subjects during surgery for traumatic lesions to the eye. Ten subjects were adults (mean age 28 years) and 10 were elderly (mean age 76 years). Morphological analysis was carried out using light microscopy and electron microscopy. Another 40 patients (20 young: mean age < 30 years; 20 elderly: mean age > 70 years) were studied in vivo by confocal microscopy. The resulting images were analyzed qualitatively, quantitatively, and statistically. The basic light microscope revealed a decrease in endothelial cell density with age accompanied by an increase in endothelial cell size. Transmission electron microscopy revealed a corneal thinning and a decrease in the number of corneal stromal cells. A marked decrease in stromal nerve fibers was observed in the older subjects compared to the younger ones. Variable pressure scanning electron microscopy (VP-SEM) was used to make surface morphological observations and to determine the chemical composition of in vivo hydrated human corneas. Our results showed the effects of aging on normal corneal morphology highlighting the structural diversity of the corneal layers and revealing an age-related reduction in nerve fibers, thus explaining the decreased corneal sensitivity that may be observed in the elderly. Clin. Anat. 33:245-256, 2020. © 2019 Wiley Periodicals, Inc.

Indexed as

Age FactorsAdultAgedAged, 80 and overCell CountCorneaFemaleFormaldehydeHumansMaleMicroscopy, ConfocalMicroscopy, ElectronNerve FibersFormaldehydeage-related changescorneaICVMlight microscopenerve fibersTEMVP-SEM

Identifiers

PMID31595552
PMCPMC7027767
OpenAlexW2980279573

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.