ArticleJournal of industrial microbiology & biotechnology2019
Beneficial mutations for carotenoid production identified from laboratory-evolved Saccharomyces cerevisiae.
Article in Journal of industrial microbiology & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 15 citations in OpenAlex.
- Metabolic engineering and adaptive laboratory evolution enhance squalene production inSynthetic and systems biotechnology · 2026Article
- Evaluating Limits of Machine Learning-Assisted Raman Spectroscopy in Classification of Biological Samples.ACS omega · 2026Article
- Evaluating Limits of Machine Learning-Assisted Raman Spectroscopy in Classification of Biological Samples.bioRxiv : the preprint server for biology · 2026Article
- A discussion and evaluation of statistical procedures used by JIMB authors when comparing means.Journal of industrial microbiology & biotechnology · 2024Article
- Advances in the Discovery and Engineering of Gene Targets for Carotenoid Biosynthesis in Recombinant Strains.Biomolecules · 2023Review
- Industrially Important Fungal Carotenoids: Advancements in Biotechnological Production and Extraction.Journal of fungi (Basel, Switzerland) · 2023Review
- Transcriptomics analysis and fed-batch regulation of high astaxanthin-producing Phaffia rhodozyma/Xanthophyllomyces dendrorhous obtained through adaptive laboratory evolution.Journal of industrial microbiology & biotechnology · 2023Article
- Carotenoids and Their Biosynthesis in Fungi.Molecules (Basel, Switzerland) · 2022Review
- Identification of a novel metabolic engineering target for carotenoid production in Saccharomyces cerevisiae via ethanol-induced adaptive laboratory evolution.Bioresources and bioprocessing · 2021Article
- Carotenoids and Some Other Pigments from Fungi and Yeasts.Metabolites · 2021Review
- Metabolic engineering of Saccharomyces cerevisiae for production of β-carotene from hydrophobic substrates.FEMS yeast research · 2021Article
- Adaptive laboratory evolution for growth coupled microbial production.World journal of microbiology & biotechnology · 2020Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Adaptive laboratory evolution (ALE) is a powerful tool used to increase strain fitness in the presence of environmental stressors. If production and strain fitness can be coupled, ALE can be used to increase product formation. In earlier work, carotenoids hyperproducing mutants were obtained using an ALE strategy. Here, de novo mutations were identified in hyperproducers, and reconstructed mutants were explored to determine the exact impact of each mutation on production and tolerance. A single mutation in YMRCTy1-3 conferred increased carotenoid production, and when combined with other beneficial mutations led to further increased β-carotene production. Findings also suggest that the ALE strategy selected for mutations that confer increased carotenoid production as primary phenotype. Raman spectroscopy analysis and total lipid quantification revealed positive correlation between increased lipid content and increased β-carotene production. Finally, we demonstrated that the best combinations of mutations identified for β-carotene production were also beneficial for production of lycopene.
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