Evidence map›Paper›PMID 31595394›Full record

ArticleNeurocritical care2020

mRNA Expression Profiles from Whole Blood Associated with Vasospasm in Patients with Subarachnoid Hemorrhage.

Huichun Xu, Boryana Stamova, Bradley P Ander, Ben Waldau, Glen C Jickling, Frank R Sharp, Nerissa U Ko

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurocritical care, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 14 citations in OpenAlex.

  1. Molecular heterogeneity in human stroke - What can we learn from the peripheral blood transcriptome?Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Huichun Xu *Department of Medicine, University of Maryland, College Park, USA.
Boryana Stamova *Department of Neurology, University of California at Davis, 2805 50th St., Sacramento, CA, 95817, USA.
Bradley P AnderDepartment of Neurology, University of California at Davis, 2805 50th St., Sacramento, CA, 95817, USA.
Ben WaldauNeurosurgery, University of California at Davis, Sacramento, USA.
Glen C JicklingDepartment of Neurology, University of California at Davis, 2805 50th St., Sacramento, CA, 95817, USA.
Frank R SharpDepartment of Neurology, University of California at Davis, 2805 50th St., Sacramento, CA, 95817, USA. frsharp@ucdavis.edu.ORCID http://orcid.org/0000-0002-5642-1459
Nerissa U KoDepartment of Neurology, University of California at San Francisco, San Francisco, USA.
University of California, Davis · USUniversity of Alberta · CAUniversity of California, San Francisco · USUniversity of Maryland, College Park · US

Funding

Molecular Biology of Stroke in HumansR01NS075035 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SHARP, FRANK R · 2012 to 2016
$3.1M
Whole Genome RNA Sequencing (RNAseq) of Blood from Patients with Lacunar StrokeR01NS101718 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SHARP, FRANK R, STAMOVA, BORYANA · 2017 to 2021
$3.0M
Whole Transcriptome Studies of Patients with Transient Ischemic Attacks (TIAs)R01NS097000 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SHARP, FRANK R, STAMOVA, BORYANA · 2016 to 2020
$2.8M
NINDS NIH HHS R01 NS075035NINDS NIH HHS R01 NS097000NINDS NIH HHS R01 NS101718
6 · The paper itself

Abstract

backgroundThough there are many biomarker studies of plasma and serum in patients with aneurysmal subarachnoid hemorrhage (SAH), few have examined blood cells that might contribute to vasospasm. In this study, we evaluated inflammatory and prothrombotic pathways by examining mRNA expression in whole blood of SAH patients with and without vasospasm.

methodsAdult SAH patients with vasospasm (n = 29) and without vasospasm (n = 21) were matched for sex, race/ethnicity, and aneurysm treatment method. Diagnosis of vasospasm was made by angiography. mRNA expression was measured by Affymetrix Human Exon 1.0 ST Arrays. SAH patients with vasospasm were compared to those without vasospasm by ANCOVA to identify differential gene, exon, and alternatively spliced transcript expression. Analyses were adjusted for age, batch, and time of blood draw after SAH.

resultsAt the gene level, there were 259 differentially expressed genes between SAH patients with vasospasm compared to patients without (false discovery rate < 0.05, |fold change| ≥ 1.2). At the exon level, 1210 exons representing 1093 genes were differentially regulated between the two groups (P < 0.005, ≥ 1.2 |fold change|). Principal components analysis segregated SAH patients with and without vasospasm. Signaling pathways for the 1093 vasospasm-related genes included adrenergic, P2Y, ET-1, NO, sildenafil, renin-angiotensin, thrombin, CCR3, CXCR4, MIF, fMLP, PKA, PKC, CRH, PPARα/RXRα, and calcium. Genes predicted to be alternatively spliced included IL23A, RSU1, PAQR6, and TRIP6.

conclusionsThis is the first study to demonstrate that mRNA expression in whole blood distinguishes SAH patients with vasospasm from those without vasospasm and supports a role of coagulation and immune systems in vasospasm.

Indexed as

AdultAgedAneurysm, RupturedFemaleHumansIntracranial AneurysmMaleMiddle AgedPrincipal Component AnalysisRNA, MessengerSubarachnoid HemorrhageTranscriptomeVasospasm, IntracranialRNA, MessengerAneurysmDelayed cerebral ischemia (DCI)MicroarrayRNASubarachnoid hemorrhageVasospasm

Identifiers

PMID31595394
PMCPMC7392923
OpenAlexW2980116194

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.