ArticleScientific reports2019
Improvement of the Pacific bluefin tuna (Thunnus orientalis) reference genome and development of male-specific DNA markers.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed.
- Evidence for <italic>sult1st6y</italic> as a Major Candidate Sex-Determining Gene in Pacific Bluefin Tuna.Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation · 2026Article
- Distribution of estrogen sulfotransferase genes in tunas and their relatives.BMC research notes · 2025Article
- Five millennia of mitonuclear discordance in Atlantic bluefin tuna identified using ancient DNA.Heredity · 2025Article
- Chromosome-level genome assembly of Indo-Pacific king mackerel (Scomberomorus guttatus).Scientific data · 2024Article
- Article
- Identification of sex-specific markers using genome re-sequencing in the blunt snout bream (Megalobrama amblycephala).BMC genomics · 2024Article
- Genetic Sex and Origin Identification Suggests Differential Migration of Male and Female Atlantic Bluefin Tuna (Evolutionary applications · 2024Article
- Genomic resources for the Yellowfin tuna Thunnus albacares.Molecular biology reports · 2024Article
- Identification of Sex-Specific Markers and Candidate Genes Using WGS Sequencing Reveals a ZW-Type Sex-Determination System in the Chinese Soft-Shell Turtle (International journal of molecular sciences · 2024Article
- Transcriptome characterization of gonadal sex differentiation in Pacific bluefin tuna, Thunnus orientalis (Temminck et Schlegel).Scientific reports · 2023Article
- The genetic basis of the leafy seadragon's unique camouflage morphology and avenues for its efficient conservation derived from habitat modeling.Science China. Life sciences · 2023Article
- Chromosome-level genome assembly of largemouth bass (Micropterus salmoides) using PacBio and Hi-C technologies.Scientific data · 2022Article
- The First Genome Survey and De Novo Assembly of the Short Mackerel (Animals : an open access journal from MDPI · 2022Article
- Sex-Specific Genomic Region Identification and Molecular Sex Marker Development of Rock Bream (Oplegnathus fasciatus).Marine biotechnology (New York, N.Y.) · 2022Article
- A genome assembly of the Atlantic chub mackerel (GigaByte (Hong Kong, China) · 2022Article
- Whole genome sequencing of the fast-swimming Southern bluefin tuna (Frontiers in genetics · 2022Article
- Article
- Adaptive markers distinguish North and South Pacific Albacore amid low population differentiation.Evolutionary applications · 2021Article
- Updated Genome Assembly of Bighead Carp (Frontiers in genetics · 2021Article
- Prediction of the Sex-Associated Genomic Region in Tunas (International journal of genomics · 2021Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The Pacific bluefin tuna, Thunnus orientalis, is a highly migratory species that is widely distributed in the North Pacific Ocean. Like other marine species, T. orientalis has no external sexual dimorphism; thus, identifying sex-specific variants from whole genome sequence data is a useful approach to develop an effective sex identification method. Here, we report an improved draft genome of T. orientalis and male-specific DNA markers. Combining PacBio long reads and Illumina short reads sufficiently improved genome assembly, with a 38-fold increase in scaffold contiguity (to 444 scaffolds) compared to the first published draft genome. Through analysing re-sequence data of 15 males and 16 females, 250 male-specific SNPs were identified from more than 30 million polymorphisms. All male-specific variants were male-heterozygous, suggesting that T. orientalis has a male heterogametic sex-determination system. The largest linkage disequilibrium block (3,174 bp on scaffold_064) contained 51 male-specific variants. PCR primers and a PCR-based sex identification assay were developed using these male-specific variants. The sex of 115 individuals (56 males and 59 females; sex was diagnosed by visual examination of the gonads) was identified with high accuracy using the assay. This easy, accurate, and practical technique facilitates the control of sex ratios in tuna farms. Furthermore, this method could be used to estimate the sex ratio and/or the sex-specific growth rate of natural populations.
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