ArticleACS nano2020
Engineering an Enzyme for Direct Electrical Monitoring of Activity.
Article in ACS nano, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Quantifying the Characteristic Turnover Frequency of an Individual Redox Enzyme via Real-Time Electrical Transduction of Catalytic Events in a Label-Free Single-Protein Junction.Journal of the American Chemical Society · 2026Article
- Real-time electrical monitoring of enzymatic catalytic dynamics at the single-molecule level.Nature communications · 2026Article
- Single-Molecule Conductance of Non-Redox Proteins: Mechanisms, Measurements, and Applications.Biomolecules · 2026Review
- Article
- DNA-Guided Robust Single-Protein Electronic Readout.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Conducting Atomic Force Microscopy of Protein Wires.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Scanning Tunneling Microscope Measurement of Proteasome Conductance.Biomolecules · 2025Article
- Electromigrated Gold Nanogap Tunnel Junction Arrays: Fabrication and Electrical Behavior in Liquid and Gaseous Media.ACS applied materials & interfaces · 2024Article
- Single-Molecule Electrical Profiling of Peptides and Proteins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Heavy Water Reduces the Electronic Conductance of Protein Wires via Deuteron Interactions with Aromatic Residues.Nano letters · 2023Article
- Fabrication of electron tunneling probes for measuring single-protein conductance.Nature protocols · 2023Review
- Advances in single-molecule junctions as tools for chemical and biochemical analysis.Nature chemistry · 2023Review
- SARS-CoV-2 spike proteins react with Au and Si, are electrically conductive and denature at 3 × 10Chemical science · 2023Article
- Biotin Binding Hardly Affects Electron Transport Efficiency across Streptavidin Solid-State Junctions.Langmuir : the ACS journal of surfaces and colloids · 2023Article
- Measuring conductance switching in single proteins using quantum tunneling.Science advances · 2022Article
- Molecular electronics sensors on a scalable semiconductor chip: A platform for single-molecule measurement of binding kinetics and enzyme activity.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Probing Bioelectronic Connections Using Streptavidin Molecules with Modified Valency.Journal of the American Chemical Society · 2021Article
- The emerging landscape of single-molecule protein sequencing technologies.Nature methods · 2021Review
- Ubiquitous Electron Transport in Non-Electron Transfer Proteins.Life (Basel, Switzerland) · 2020Review
- Electronic Conductance Resonance in Non-Redox-Active Proteins.Journal of the American Chemical Society · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Proteins have been shown to be electrically conductive if tethered to an electrode by means of a specific binding agent, allowing single molecules to be wired into an electrical sensing circuit. Such circuits allow enzymes to be used as sensors, detectors, and sequencing devices. We have engineered contact points into a Φ29 polymerase by introducing biotinylatable peptide sequences. The modified enzyme was bound to electrodes functionalized with streptavidin. Φ29 connected by one biotinylated contact, and a second nonspecific contact showed rapid small fluctuations in current when activated. Signals were greatly enhanced with two specific contacts. Features in the distributions of DC conductance increased by a factor 2 or more over the open to closed conformational transition of the polymerase. Polymerase activity is manifested by a rapid (millisecond) large (25% of background) current fluctuations imposed on the DC conductance.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.