Evidence map›Paper›PMID 31592172›Full record

ArticlePeerJ2019

An application of PCR-RFLP species identification assay for environmental DNA detection.

Takeshi Igawa, Teruhiko Takahara, Quintin Lau, Shohei Komaki

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. An overview of remote monitoring methods in biodiversity conservation.Environmental science and pollution research international · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Takeshi IgawaAmphibian Research Center, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan.ORCID 0000-0001-6880-6180
Teruhiko TakaharaFaculty of Life and Environmental Science, Shimane University, Matsue, Shimane, Japan.
Quintin LauDepartment of Evolutionary Studies of Biosystems, Sokendai (The Graduate University for Advanced Studies), Hayama, Kanagawa, Japan.ORCID 0000-0002-6716-4515
Shohei KomakiDivision of Biomedical Information Analysis, Iwate Tohoku Medical Megabank Organization, Disaster Reconstruction Center, Iwate Medical University, Shiwa, Iwate, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recent advancement of environmental DNA (eDNA) methods for surveying species in aquatic ecosystems has been used for various organisms and contributed to monitoring and conservation of species and environments. Amphibians are one of the promising taxa which could be monitored efficiently by applying quantitative PCR (qPCR) or next generation sequencing to eDNA. However, the cost of eDNA detection using these approaches can be quite high and requires instruments that are not usually installed in ecology laboratories. For aiding researchers in starting eDNA studies of amphibians, especially those not specialized in molecular biology, we developed a cost efficient protocol using PCR-RFLP method. We attempted to detect eDNA of three Japanese

Indexed as

AmphibianDistribution rangeEnvironmental DNAFrog speciesMonitoring surveySpecies identificationTadpoleWater sample

Identifiers

PMID31592172
PMCPMC6778664

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.