Evidence map›Paper›PMID 31592051›Full record

ArticleJournal of clinical biochemistry and nutrition2019

Analysis of the colonic mucosa associated microbiota (MAM) using brushing samples during colonic endoscopic procedures.

Hiroshi Matsumoto, Yasutoshi Kuroki, Seiya Higashi, Kyosuke Goda, Shinya Fukushima, Ryo Katsumoto, Motoyasu Oosawa, Takahisa Murao, Manabu Ishii, Kentaro Oka and 4 more

Open access · diamondAbstract read
In one paragraph

Article in Journal of clinical biochemistry and nutrition, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 1 country.

Hiroshi MatsumotoDivision of Gastroenterology, Department of Internal Medicine, Kawasaki Medical School, Kurashiki, Okayama 710-0192, Japan.
Yasutoshi KurokiTokyo R&D Center, Miyarisan Pharmaceutical Co., Ltd., Tokyo 114-0016, Japan.
Seiya HigashiTokyo R&D Center, Miyarisan Pharmaceutical Co., Ltd., Tokyo 114-0016, Japan.
Kyosuke GodaDivision of Gastroenterology, Department of Internal Medicine, Kawasaki Medical School, Kurashiki, Okayama 710-0192, Japan.
Shinya FukushimaDivision of Gastroenterology, Department of Internal Medicine, Kawasaki Medical School, Kurashiki, Okayama 710-0192, Japan.
Ryo KatsumotoDivision of Gastroenterology, Department of Internal Medicine, Kawasaki Medical School, Kurashiki, Okayama 710-0192, Japan.
Motoyasu OosawaDivision of Gastroenterology, Department of Internal Medicine, Kawasaki Medical School, Kurashiki, Okayama 710-0192, Japan.
Takahisa MuraoDivision of Gastroenterology, Department of Internal Medicine, Kawasaki Medical School, Kurashiki, Okayama 710-0192, Japan.
Manabu IshiiDivision of Gastroenterology, Department of Internal Medicine, Kawasaki Medical School, Kurashiki, Okayama 710-0192, Japan.
Kentaro OkaTokyo R&D Center, Miyarisan Pharmaceutical Co., Ltd., Tokyo 114-0016, Japan.
Motomichi TakahashiTokyo R&D Center, Miyarisan Pharmaceutical Co., Ltd., Tokyo 114-0016, Japan.
Takako OsakiDepartment of Infectious Diseases, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan.
Shigeru KamiyaDepartment of Health Science, Kyorin University Faculty of Health Sciences, Tokyo 181-8611, Japan.
Akiko ShiotaniDivision of Gastroenterology, Department of Internal Medicine, Kawasaki Medical School, Kurashiki, Okayama 710-0192, Japan.
Kawasaki Medical School · JPKyorin University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The mucosa-associated microbiota is an important component in human microbiota. The aim was to investigate mucosa-associated microbiota using brush samples during endoscopic procedures and compare with fecal microbiota. Seven patients who were planning to undergo a routine colonoscopy were recruited. Mucosal brushing samples were taken from 3 sites (terminal ileum, ascending and sigmoid colon), and a fecal sample was taken on the morning of colonoscopy. The samples were immediately placed in microcentrifuge tubes containing DNA stabilization reagent and analyzed using the next generation sequencer. The individual differences in microbiota were more evident than the differences of the sampling sites. Actinobacteria was more abundant and Bacteroidetes was less in the brush samples than those in the fecal samples. Taxonomic composition at the genus level and the proportion of genes responsible for some functions in the brushing samples tended to be different from those in the fecal samples.

Indexed as

16S rRNA sequencingbrushing samplescolonic mucusmucosa associated microbiota (MAM)taxonomic composition

Identifiers

PMID31592051
PMCPMC6769413
OpenAlexW2964984261

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.