ArticleAPL bioengineering2019
Varying solvent type modulates collagen coating and stem cell mechanotransduction on hydrogel substrates.
Article in APL bioengineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Protocol for synthesis and mechanical characterization of polyacrylamide hydrogels of varying stiffness for cell culture applications.STAR protocols · 2026Article
- Deciphering Stiffness-Driven Changes in Colorectal Cancer by Proteomics.Molecular & cellular proteomics : MCP · 2026Article
- Flow-dependent traction in high- and low-flow normal trabecular meshwork cells.Acta biomaterialia · 2026Article
- Linking molecular tension and cellular tractions: a multiscale approach to focal adhesion mechanics.Communications biology · 2026Article
- Self-assembly of short biopeptides onto skin tissue components studied using QCM-D.International journal of cosmetic science · 2025Article
- Enhanced PDMS Functionalization for Organ-on-a-Chip Platforms Using Ozone and Sulfo-SANPAH: A Simple Approach for Biomimetic Long-Term Cell Cultures.Advanced healthcare materials · 2025Article
- Review
- Dynamic and Reversible Tuning of Hydrogel Viscoelasticity by Transient Polymer Interactions for Controlling Cell Adhesion.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Hydrogels and nanogels: effectiveness in dermal applications.Beilstein journal of nanotechnology · 2025Review
- Vascularized tumor models for the evaluation of drug delivery systems: a paradigm shift.Drug delivery and translational research · 2024Review
- Active Media Perfusion in Bioprinted Highly Concentrated Collagen Bioink Enhances the Viability of Cell Culture and Substrate Remodeling.Gels (Basel, Switzerland) · 2024Article
- Modulation of Stiffness-Dependent Macrophage Inflammatory Responses by Collagen Deposition.ACS biomaterials science & engineering · 2024Article
- Regulation of cell attachment, spreading, and migration by hydrogel substrates with independently tunable mesh size.Acta biomaterialia · 2022Article
- An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells.Journal of visualized experiments : JoVE · 2021Article
- Mimicking the Hierarchical Organization of Natural Collagen: Toward the Development of Ideal Scaffolding Material for Tissue Regeneration.Frontiers in bioengineering and biotechnology · 2021Review
- Biopolymer Coatings for Biomedical Applications.Polymers · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Type I collagen is the most abundant extracellular matrix protein in the human body and is commonly used as a biochemical ligand for hydrogel substrates to support cell adhesion in mechanotransduction studies. Previous protocols for conjugating collagen I have used different solvents; yet, how varying solvent pH and composition impacts the efficiency and distribution of these collagen I coatings remains unknown. Here, we examine the effect of varying solvent pH and type on the efficiency and distribution of collagen I coatings on polyacrylamide hydrogels. We further evaluate the effects of varying solvent on mechanotransduction of human mesenchymal stem cells (MSCs) by characterizing cell spreading and localization of Yes-Associated Protein (YAP), a key transcriptional regulator of mechanotransduction. Increasing solvent pH to 5.2 and above increased the heterogeneity of coating with collagen bundle formation. Collagen I coating highly depends on the solvent type, with acetic acid leading to the highest conjugation efficiency and most homogeneous coating. Compared to HEPES or phosphate-buffered saline buffer, acetic acid-dissolved collagen I coatings substantially enhance MSC adhesion and spreading on both glass and polyacrylamide hydrogel substrates. When acetic acid was used for collagen coatings, even the low collagen concentration (1
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.