ArticleJournal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie2020
Regulation of the autophagy-marker Sequestosome 1 in periodontal cells and tissues by biomechanical loading.
Article in Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 15 citations in OpenAlex.
- Association Between Single-Nucleotide Polymorphisms and DRAM1 Gene Expression in Periodontal Ligament Fibroblasts Under Orthodontic Compression.Biomedicines · 2026Article
- Cell Death in Orthodontic Tooth Movement: Recent Advances and Emerging Insights.International journal of molecular sciences · 2026Review
- From mechanical force to cellular response: the role of autophagy in orthodontic tooth movement.Frontiers in physiology · 2026Review
- Autophagy in orthodontic tooth movement: advances, challenges, and future perspectives.Molecular medicine (Cambridge, Mass.) · 2025Review
- Cyclic mechanical strain with high-tensile triggers autophagy in growth plate chondrocytes.Journal of orthopaedic surgery and research · 2022Article
- Role of chaperone-assisted selective autophagy (CASA) in mechanical stress protection of periodontal ligament cells.Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie · 2022Article
- Biomechanical and biological responses of periodontium in orthodontic tooth movement: up-date in a new decade.International journal of oral science · 2021Review
- Dynamic study into autophagy and apoptosis during orthodontic tooth movement.Experimental and therapeutic medicine · 2021Article
- Article
- Effect of Tension on Human Periodontal Ligament Cells: Systematic Review and Network Analysis.Frontiers in bioengineering and biotechnology · 2021Article
- Autophagy Induces Expression of IL-6 in Human Periodontal Ligament Fibroblasts Under Mechanical Load and Overload and Effects OsteoclastogenesisFrontiers in physiology · 2021Article
- Regulation of Autophagic Signaling by Mechanical Loading and Inflammation in Human PDL Fibroblasts.International journal of molecular sciences · 2020Article
- A Human Periodontal Ligament Fibroblast Cell Line as a New Model to Study Periodontal Stress.International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeOrthodontic treatment is based on the principle of force application to teeth and subsequently to the surrounding tissues and periodontal cells. Sequestosome 1 (SQSTM1) is a well-known marker for autophagy, which is an important cellular mechanism of adaptation to stress. The aim of this study was to analyze whether biomechanical loading conditions regulate SQSTM1 in periodontal cells and tissues, thereby providing further information on the role of autophagy in orthodontic tooth movement.
methodsPeriodontal ligament (PDL) fibroblasts were exposed to cyclic tensile strain of low magnitude (3%, CTSL), and the regulation of autophagy-associated targets was determined with an array-based approach. SQSTM1 was selected for further biomechanical loading experiments with dynamic and static tensile strain and assessed via real-time polymerase chain reaction (RT-PCR) and immunoblotting. Signaling pathways involved in SQSTM1 activation were analyzed by using specific inhibitors, including an autophagy inhibitor. Finally, SQSTM1 expression was analyzed in gingival biopsies and histological sections of rats in presence and absence of orthodontic forces.
resultsMultiple autophagy-associated targets were regulated by CTSL in PDL fibroblasts. All biomechanical loading conditions tested increased the SQSTM1 expression significantly. Stimulatory effects of CTSL on SQSTM1 expression were diminished by inhibition of the c‑Jun N‑terminal kinase (JNK) pathway and of autophagy. Increased SQSTM1 levels after CTSL were confirmed by immunoblotting. Orthodontic force application also led to significantly elevated SQTSM1 levels in the gingiva and PDL of treated animals as compared to control.
conclusionsOur in vitro and in vivo findings provide evidence of a role of SQSTM1 and thereby autophagy in orthodontic tooth movement.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.