ArticleNature communications2019
Galactose to tagatose isomerization at moderate temperatures with high conversion and productivity.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Advances in the Production of D-Tagatose Using L-Arabinose Isomerase.Microorganisms · 2026Review
- Engineering ofCurrent research in food science · 2026Article
- Reversal of the Leloir pathway to promote galactose and tagatose synthesis from glucose.Cell reports. Physical science · 2025Article
- Activating the d-Tagatose Production Capacity ofJournal of agricultural and food chemistry · 2025Article
- Construction of a multienzyme cascade reaction system and its application in D-tagatose biosynthesis.AMB Express · 2025Article
- Transforming monosaccharides: Recent advances in rare sugar production and future exploration.BBA advances · 2025Article
- 2-O-α-D-glucosyl glycerol production by whole-cell biocatalyst of lactobacilli encapsulating sucrose phosphorylase with improved glycerol affinity and conversion rate.Microbial cell factories · 2024Article
- Tailoring the natural rare sugars D-tagatose and L-sorbose to produce novel functional carbohydrates.NPJ science of food · 2024Article
- Biochemical characterization, structure-guided mutagenesis, and application of a recombinant D-allulose 3-epimerase fromFrontiers in bioengineering and biotechnology · 2024Article
- Inducible directed evolution of complex phenotypes in bacteria.Nucleic acids research · 2022Article
- Sustainable isomaltulose production inFrontiers in microbiology · 2022Article
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- Improving the catalytic performance ofRSC advances · 2021Article
- An overview of D-galactose utilization through microbial fermentation and enzyme-catalyzed conversion.Applied microbiology and biotechnology · 2021Review
- Anchoring of heterologous proteins in multiple Lactobacillus species using anchors derived from Lactobacillus plantarum.Scientific reports · 2020Article
- Cell Wall Anchoring of a Bacterial Chitosanase inInternational journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
There are many industrially-relevant enzymes that while active, are severely limited by thermodynamic, kinetic, or stability issues (isomerases, lyases, transglycosidases). In this work, we study Lactobacillus sakei L-arabinose isomerase (LsLAI) for D-galactose to D-tagatose isomerization-that is limited by all three reaction parameters. The enzyme demonstrates low catalytic efficiency, low thermostability at temperatures > 40 °C, and equilibrium conversion < 50%. After exploring several strategies to overcome these limitations, we show that encapsulating LsLAI in gram-positive Lactobacillus plantarum that is chemically permeabilized enables reactions at high rates, high conversions, and elevated temperatures. In a batch process, this system enables ~ 50% conversion in 4 h starting with 300 mM galactose (an average productivity of 37 mM h
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