ArticleCell death & disease2019
Intermittent compressive force promotes osteogenic differentiation in human periodontal ligament cells by regulating the transforming growth factor-β pathway.
Article in Cell death & disease, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 57 citations in OpenAlex.
- Under pressure-mechanisms and risk factors for orthodontically induced inflammatory root resorption: a systematic review.European journal of orthodontics · 2023Pooled it
- Biomarkers for Treatment Response in Orthodontics: Molecular Mechanisms, Clinical Utility, and Future Directions.International journal of molecular sciences · 2026Review
- A 3D Collagen-Alginate Hydrogel Model for Mechanoregulation of Autophagy in Periodontal Ligament Cells.Gels (Basel, Switzerland) · 2026Article
- Substrate stiffness modulates human gingival fibroblast paracrine signaling to promote osteogenic differentiation of human periodontal ligament cells.Frontiers in bioengineering and biotechnology · 2026Article
- Mapping Immune-Inflammatory Niches on Zirconia Bone Implants: Single-Cell Transcriptomic Profiling.Research (Washington, D.C.) · 2026Article
- Periodontal ligament stem cells in tissue remodeling: from mechanical forces to inflammatory signals.Stem cell research & therapy · 2025Review
- Multi-omics approach reveals TGF-β signaling-driven senescence in periodontium stem cells.Journal of advanced research · 2025Article
- Decellularised matrices from force loaded periodontal ligament stem cells support osteogenic differentiation.Scientific reports · 2025Article
- Decellularised matrices from force loaded periodontal ligament stem cells support osteogenic differentiation.Scientific reports · 2025Article
- Shear stress-triggered adenosine triphosphate regulates angiogenic properties of human periodontal ligament stem cells.Scientific reports · 2025Article
- Autophagy in orthodontic tooth movement: advances, challenges, and future perspectives.Molecular medicine (Cambridge, Mass.) · 2025Review
- Osteogenic Induction Activity of Magnesium Chloride on Human Periodontal Ligament Stem Cells.International dental journal · 2025Article
- Mechanical force modulates inflammation and immunomodulation in periodontal ligament cells.Medical review (2021) · 2024Article
- Revealing the Potency of Growth Factors in Bovine Colostrum.Nutrients · 2024Review
- Involvement of RAMP1/p38MAPK signaling pathway in osteoblast differentiation in response to mechanical stimulation: a preliminary study.Journal of orthopaedic surgery and research · 2024Article
- Mechanochemical coupling of MGF mediates periodontal regeneration.Bioengineering & translational medicine · 2024Article
- Alteration of extracellular matrix proteins in atrophic periodontal ligament of hypofunctional rat molars.BDJ open · 2023Article
- Roles of extracellular adenosine triphosphate on the functions of periodontal ligament cells.BDJ open · 2023Review
- Dissecting specific Wnt components governing osteogenic differentiation potential by human periodontal ligament stem cells through interleukin-6.Scientific reports · 2023Article
- New strategy of personalized tissue regeneration: when autologous platelet concentrates encounter biomaterials.Frontiers in bioengineering and biotechnology · 2023Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mechanical force regulates periodontal ligament cell (PDL) behavior. However, different force types lead to distinct PDL responses. Here, we report that pretreatment with an intermittent compressive force (ICF), but not a continuous compressive force (CCF), promoted human PDL (hPDL) osteogenic differentiation as determined by osteogenic marker gene expression and mineral deposition in vitro. ICF-induced osterix (OSX) expression was inhibited by cycloheximide and monensin. Although CCF and ICF significantly increased extracellular adenosine triphosphate (ATP) levels, pretreatment with exogenous ATP did not affect hPDL osteogenic differentiation. Gene-expression profiling of hPDLs subjected to CCF or ICF revealed that extracellular matrix (ECM)-receptor interaction, focal adhesion, and transforming growth factor beta (TGF-β) signaling pathway genes were commonly upregulated, while calcium signaling pathway genes were downregulated in both CCF- and ICF-treated hPDLs. The TGFB1 mRNA level was significantly increased, while those of TGFB2 and TGFB3 were decreased by ICF treatment. In contrast, CCF did not modify TGFB1 expression. Inhibiting TGF-β receptor type I or adding a TGF-β1 neutralizing antibody attenuated the ICF-induced OSX expression. Exogenous TGF-β1 pretreatment promoted hPDL osteogenic marker gene expression and mineral deposition. Additionally, pretreatment with ICF in the presence of TGF-β receptor type I inhibitor attenuated the ICF-induced mineralization. In conclusion, this study reveals the effects of ICF on osteogenic differentiation in hPDLs and implicates TGF-β signaling as one of its regulatory mechanisms.
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