Evidence map›Paper›PMID 31591262›Full record

ArticleMolecular & cellular proteomics : MCP2020

NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.

Maria Lorna A De Leoz, David L Duewer, Adam Fung, Lily Liu, Hoi Kei Yau, Oscar Potter, Gregory O Staples, Kenichiro Furuki, Ruth Frenkel, Yunli Hu and 152 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular & cellular proteomics : MCP, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.

0numbers the graph read from it
0cells of the map it votes in
58citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

58 citing papers in PubMed.

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  13. Liquid Biopsy Proteomics in Ophthalmology.Journal of proteome research · 2024
    Review
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  16. ErythropoietinBiomolecules · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

162 authors.

Maria Lorna A De LeozMass Spectrometry Data Center, Biomolecular Measurement Division, Material Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive Gaithersburg, Maryland 20899. Electronic address: lornadeleoz@gmail.com.
David L DuewerChemical Sciences Division, Material Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive Gaithersburg, Maryland 20899.
Adam FungAnalytical Development, Agensys, Inc., 1800 Steward Street Santa Monica, California 90404.
Lily LiuAnalytical Development, Agensys, Inc., 1800 Steward Street Santa Monica, California 90404.
Hoi Kei YauAnalytical Development, Agensys, Inc., 1800 Steward Street Santa Monica, California 90404.
Oscar PotterAgilent Technologies, Inc., 5301 Stevens Creek Blvd Santa Clara, California 95051.
Gregory O StaplesAgilent Technologies, Inc., 5301 Stevens Creek Blvd Santa Clara, California 95051.
Kenichiro FurukiAstellas Pharma, 5-2-3 Tokodai, Tsukiba, Ibaraki, 300-2698, Japan.
Ruth FrenkelAnalytical Development, Biogen, 14 Cambridge Center Cambridge, Massachusetts 02142.
Yunli HuAnalytical Development, Biogen, 14 Cambridge Center Cambridge, Massachusetts 02142.
Zoran SosicAnalytical Development, Biogen, 14 Cambridge Center Cambridge, Massachusetts 02142.
Peiqing ZhangBioprocessing Technology Institute, 20 Biopolis Way, Level 3 Singapore 138668.
Friedrich AltmannDepartment of Chemistry, University of Natural Resources and Life Science, Vienna (BOKU), Muthgasse 18 1190 Wien, Austria.
Clemens Grunwald-GrubeDepartment of Chemistry, University of Natural Resources and Life Science, Vienna (BOKU), Muthgasse 18 1190 Wien, Austria.
Chun ShaoCenter for Biomedical Mass Spectrometry, Boston University School of Medicine, 670 Albany Street Boston, Massachusetts 02118.
Joseph ZaiaCenter for Biomedical Mass Spectrometry, Boston University School of Medicine, 670 Albany Street Boston, Massachusetts 02118.
Waltraud EversBruker Daltonik GmbH, Fahrenheitstr. 4, 28359 Bremen, Germany.
Stuart PengelleyBruker Daltonik GmbH, Fahrenheitstr. 4, 28359 Bremen, Germany.
Detlev SuckauBruker Daltonik GmbH, Fahrenheitstr. 4, 28359 Bremen, Germany.
Anja WiechmannBruker Daltonik GmbH, Fahrenheitstr. 4, 28359 Bremen, Germany.
Anja ResemannBruker Daltonik GmbH, Fahrenheitstr. 4, 28359 Bremen, Germany.
Wolfgang JabsBruker Daltonik GmbH, Fahrenheitstr. 4, 28359 Bremen, Germany; Department of Life Sciences & Technology, Beuth Hochschule für Technik Berlin, Seestraβe 64, 13347 Berlin, Germany.
Alain BeckCentre d'Immunologie Pierre Fabre, 5 Avenue Napoléon III, BP 60497, 74164 St Julien-en-Genevois, France.
John W FroehlichDepartment of Urology, Boston Children's Hospital, 300 Longwood Avenue Boston Massachusetts 02115.
Chuncui HuangInstitute of Biophysics, Chinese Academy of Sciences, 15 Da Tun Road, Chaoyang District, Beijing 100101 China.
Yan LiInstitute of Biophysics, Chinese Academy of Sciences, 15 Da Tun Road, Chaoyang District, Beijing 100101 China.
Yaming LiuInstitute of Biophysics, Chinese Academy of Sciences, 15 Da Tun Road, Chaoyang District, Beijing 100101 China.
Shiwei SunKey Lab of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences, 15 Da Tun Road, Chaoyang District, Beijing 100101 China.
Yaojun WangKey Lab of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences, 15 Da Tun Road, Chaoyang District, Beijing 100101 China.
Youngsuk SeoGraduate School of Analytical Science and Technology, Chungnam National University, Gung-dong 220, Yuseong-Gu, Daejeon 305-764, Korea (South).
Hyun Joo AnGraduate School of Analytical Science and Technology, Chungnam National University, Gung-dong 220, Yuseong-Gu, Daejeon 305-764, Korea (South).
Niels-Christian ReichardtCICbiomaGUNE, Paseo Miramon 182, 20009 San Sebastian, Spain.
Juan Echevarria RuizCICbiomaGUNE, Paseo Miramon 182, 20009 San Sebastian, Spain.
Stephanie Archer-HartmannAnalytical Services, Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road Athens, Georgia 30602.
Parastoo AzadiAnalytical Services, Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road Athens, Georgia 30602.
Len BellBioCMC Solutions (Large Molecules), Covance Laboratories Limited, Otley Road, Harrogate, North Yorks HG3 1PY, United Kingdom.
Zsuzsanna LakosBiochemistry Method Development & Validation, Eurofins Lancaster Laboratories, Inc., 2425 New Holland Pike Lancaster, Pennsylvania 17601.
Yanming AnCenter for Biologics Evaluation and Research, Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, Maryland 20993.
John F CipolloCenter for Biologics Evaluation and Research, Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, Maryland 20993.
Maja Pucic-BakovicGlycoscience Research Laboratory, Genos, Borongajska cesta 83h, 10 000 Zagreb, Croatia.
Jerko ŠtambukGlycoscience Research Laboratory, Genos, Borongajska cesta 83h, 10 000 Zagreb, Croatia.
Gordan LaucGlycoscience Research Laboratory, Genos, Borongajska cesta 83h, 10 000 Zagreb, Croatia; Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, 10 000 Zagreb, Croatia.
Xu LiDepartment of Chemistry, Georgia State University, 100 Piedmont Avenue, Atlanta, Georgia 30303.
Peng George WangDepartment of Chemistry, Georgia State University, 100 Piedmont Avenue, Atlanta, Georgia 30303.
Andreas BockglyXera GmbH, Brenneckestrasse 20 * ZENIT / 39120 Magdeburg, Germany.
René HennigglyXera GmbH, Brenneckestrasse 20 * ZENIT / 39120 Magdeburg, Germany.
Erdmann RappglyXera GmbH, Brenneckestrasse 20 * ZENIT / 39120 Magdeburg, Germany; AstraZeneca, Granta Park, Cambridgeshire, CB21 6GH United Kingdom.
Marybeth CreskeyHealth Products and Foods Branch, Health Canada, AL 2201E, 251 Sir Frederick Banting Driveway, Ottawa, Ontario, K1A 0K9 Canada.
Terry D CyrHealth Products and Foods Branch, Health Canada, AL 2201E, 251 Sir Frederick Banting Driveway, Ottawa, Ontario, K1A 0K9 Canada.
Miyako NakanoGraduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama Higashi-Hiroshima 739-8530 Japan.
Taiki SugiyamaGraduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama Higashi-Hiroshima 739-8530 Japan.
Pui-King Amy LeungImmunoGen, 830 Winter Street, Waltham, Massachusetts 02451.
Paweł Link-LenczowskiDepartment of Medical Physiology, Jagiellonian University Medical College, ul. Michalowskiego 12, 31-126 Krakow, Poland.
Jolanta JaworekDepartment of Medical Physiology, Jagiellonian University Medical College, ul. Michalowskiego 12, 31-126 Krakow, Poland.
Shuang YangDepartment of Pathology, Johns Hopkins University, 400 N. Broadway Street Baltimore, Maryland 21287.
Hui ZhangDepartment of Pathology, Johns Hopkins University, 400 N. Broadway Street Baltimore, Maryland 21287.
Tim KellyMass Spec Core Facility, KBI Biopharma, 1101 Hamlin Road Durham, North Carolina 27704.
Song KlapoetkeMass Spec Core Facility, KBI Biopharma, 1101 Hamlin Road Durham, North Carolina 27704.
Rui CaoMass Spec Core Facility, KBI Biopharma, 1101 Hamlin Road Durham, North Carolina 27704.
Jin Young KimDivision of Mass Spectrometry, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongwon-gu, Cheongju Chungbuk, 363-883 Korea (South).
Hyun Kyoung LeeDivision of Mass Spectrometry, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongwon-gu, Cheongju Chungbuk, 363-883 Korea (South).
Ju Yeon LeeDivision of Mass Spectrometry, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongwon-gu, Cheongju Chungbuk, 363-883 Korea (South).
Jong Shin YooDivision of Mass Spectrometry, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongwon-gu, Cheongju Chungbuk, 363-883 Korea (South).
Sa-Rang KimAdvanced Therapy Products Research Division, Korea National Institute of Food and Drug Safety, 187 Osongsaengmyeong 2-ro Osong-eup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do, 363-700, Korea (South).
Soo-Kyung SuhAdvanced Therapy Products Research Division, Korea National Institute of Food and Drug Safety, 187 Osongsaengmyeong 2-ro Osong-eup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do, 363-700, Korea (South).
Noortje de HaanCenter for Proteomics and Metabolomics, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.
David FalckCenter for Proteomics and Metabolomics, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.
Guinevere S M Lageveen-KammeijerCenter for Proteomics and Metabolomics, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.
Manfred WuhrerCenter for Proteomics and Metabolomics, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.
Robert J EmeryLudger Limited, Culham Science Centre, Abingdon, Oxfordshire, OX14 3EB, United Kingdom.
Radoslaw P KozakLudger Limited, Culham Science Centre, Abingdon, Oxfordshire, OX14 3EB, United Kingdom.
Li Phing LiewLudger Limited, Culham Science Centre, Abingdon, Oxfordshire, OX14 3EB, United Kingdom.
Louise RoyleLudger Limited, Culham Science Centre, Abingdon, Oxfordshire, OX14 3EB, United Kingdom.
Paulina A UrbanowiczLudger Limited, Culham Science Centre, Abingdon, Oxfordshire, OX14 3EB, United Kingdom.
Nicolle H PackerBiomolecular Discovery and Design Research Centre and ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University, North Ryde, Australia.
Xiaomin SongBiomolecular Discovery and Design Research Centre and ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University, North Ryde, Australia.
Arun Everest-DassBiomolecular Discovery and Design Research Centre and ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University, North Ryde, Australia.
Erika LattováProteomics, Central European Institute for Technology, Masaryk University, Kamenice 5, A26, 625 00 BRNO, Czech Republic.
Samanta CajicMax Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany.
Kathirvel AlagesanDepartment of Biomolecular Sciences, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Daniel KolarichDepartment of Biomolecular Sciences, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Toyin KasaliAstraZeneca, Granta Park, Cambridgeshire, CB21 6GH United Kingdom.
Viv LindoAstraZeneca, Granta Park, Cambridgeshire, CB21 6GH United Kingdom.
Yuetian ChenMerck, 2015 Galloping Hill Rd, Kenilworth, New Jersey 07033.
Kudrat GoswamiMerck, 2015 Galloping Hill Rd, Kenilworth, New Jersey 07033.
Brian GauAnalytical R&D, MilliporeSigma, 2909 Laclede Ave. St. Louis, Missouri 63103.
Ravi AmunugamaMS Bioworks, LLC, 3950 Varsity Drive Ann Arbor, Michigan 48108.
Richard JonesMS Bioworks, LLC, 3950 Varsity Drive Ann Arbor, Michigan 48108.
Corné J M StroopMSD, Molenstraat 110, 5342 CC Oss, The Netherlands.
Koichi KatoExploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787 Japan; Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuhoku, Nagoya 467-8603 Japan.
Hirokazu YagiGraduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuhoku, Nagoya 467-8603 Japan.
Sachiko KondoGraduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuhoku, Nagoya 467-8603 Japan; Medical & Biological Laboratories Co., Ltd, 2-22-8 Chikusa, Chikusa-ku, Nagoya 464-0858 Japan.
C T YuenNational Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG United Kingdom.
Akira HarazonoDivision of Biological Chemistry & Biologicals, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501 Japan.
Xiaofeng ShiNew England Biolabs, Inc., 240 County Road, Ipswich, Massachusetts 01938.
Paula E MagnelliNew England Biolabs, Inc., 240 County Road, Ipswich, Massachusetts 01938.
Brian T KasperNew York University, 100 Washington Square East New York City, New York 10003.
Lara MahalNew York University, 100 Washington Square East New York City, New York 10003.
David J HarveyTarget Discovery Institute, Nuffield Department of Medicine, University of Oxford, Roosevelt Drive, Oxford, OX3 7FZ, United Kingdom.
Roisin O'FlahertyGlycoScience Group, The National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Co. Dublin, Ireland.
Pauline M RuddGlycoScience Group, The National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Co. Dublin, Ireland.
Radka SaldovaGlycoScience Group, The National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Co. Dublin, Ireland.
Elizabeth S HechtDepartment of Chemistry, North Carolina State University, 2620 Yarborough Drive Raleigh, North Carolina 27695.
David C MuddimanDepartment of Chemistry, North Carolina State University, 2620 Yarborough Drive Raleigh, North Carolina 27695.
Jichao KangPantheon, 201 College Road East Princeton, New Jersey 08540.
Prachi BhoskarPfizer Inc., 1 Burtt Road Andover, Massachusetts 01810.
Daniele MenardPfizer Inc., 1 Burtt Road Andover, Massachusetts 01810.
Andrew SaatiPfizer Inc., 1 Burtt Road Andover, Massachusetts 01810.
Christine MerleProteodynamics, ZI La Varenne 20-22 rue Henri et Gilberte Goudier 63200 RIOM, France.
Steven MastProZyme, Inc., 3832 Bay Center Place Hayward, California 94545.
Sam TepProZyme, Inc., 3832 Bay Center Place Hayward, California 94545.
Jennie TruongProZyme, Inc., 3832 Bay Center Place Hayward, California 94545.
Takashi NishikazeKoichi Tanaka Mass Spectrometry Research Laboratory, Shimadzu Corporation, 1 Nishinokyo Kuwabara-cho Nakagyo-ku, Kyoto, 604 8511 Japan.
Sadanori SekiyaKoichi Tanaka Mass Spectrometry Research Laboratory, Shimadzu Corporation, 1 Nishinokyo Kuwabara-cho Nakagyo-ku, Kyoto, 604 8511 Japan.
Aaron ShaferChildren's GMP LLC, St. Jude Children's Research Hospital, 262 Danny Thomas Place Memphis, Tennessee 38105.
Sohei FunaokaSumitomo Bakelite Co., Ltd., 1-5 Muromati 1-Chome, Nishiku, Kobe, 651-2241 Japan.
Masaaki ToyodaSumitomo Bakelite Co., Ltd., 1-5 Muromati 1-Chome, Nishiku, Kobe, 651-2241 Japan.
Peter de VreugdSynthon Biopharmaceuticals, Microweg 22 P.O. Box 7071, 6503 GN Nijmegen, The Netherlands.
Cassie CaronTakeda Pharmaceuticals International Co., 40 Landsdowne Street Cambridge, Massachusetts 02139.
Pralima PradhanTakeda Pharmaceuticals International Co., 40 Landsdowne Street Cambridge, Massachusetts 02139.
Niclas Chiang TanTakeda Pharmaceuticals International Co., 40 Landsdowne Street Cambridge, Massachusetts 02139.
Yehia MechrefDepartment of Chemistry and Biochemistry, Texas Tech University, 2500 Broadway, Lubbock, Texas 79409.
Sachin PatilThermo Fisher Scientific, 1214 Oakmead Parkway Sunnyvale, California 94085.
Jeffrey S RohrerThermo Fisher Scientific, 1214 Oakmead Parkway Sunnyvale, California 94085.
Ranjan ChakrabartiUnited States Pharmacopeia India Pvt. Ltd. IKP Knowledge Park, Genome Valley, Shamirpet, Turkapally Village, Medchal District, Hyderabad 500 101 Telangana, India.
Disha DadkeUnited States Pharmacopeia India Pvt. Ltd. IKP Knowledge Park, Genome Valley, Shamirpet, Turkapally Village, Medchal District, Hyderabad 500 101 Telangana, India.
Mohammedazam LahoriUnited States Pharmacopeia India Pvt. Ltd. IKP Knowledge Park, Genome Valley, Shamirpet, Turkapally Village, Medchal District, Hyderabad 500 101 Telangana, India.
Chunxia ZouAlberta Glycomics Centre, University of Alberta, Edmonton, Alberta T6G 2G2 Canada; Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2 Canada.
Christopher CairoAlberta Glycomics Centre, University of Alberta, Edmonton, Alberta T6G 2G2 Canada; Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2 Canada.
Béla ReizDepartment of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2 Canada.
Randy M WhittalDepartment of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2 Canada.
Carlito B LebrillaDepartment of Chemistry, University of California, One Shields Ave, Davis, California 95616.
Lauren WuDepartment of Chemistry, University of California, One Shields Ave, Davis, California 95616.
Andras GuttmanHorváth Csaba Memorial Laboratory for Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Egyetem ter 1, Hungary.
Marton SzigetiHorváth Csaba Memorial Laboratory for Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Egyetem ter 1, Hungary; Translational Glycomics Research Group, Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Egyetem ut 10, Hungary.
Benjamin G KremkowDelaware Biotechnology Institute, University of Delaware, 15 Innovation Way Newark, Delaware 19711.
Kelvin H LeeDelaware Biotechnology Institute, University of Delaware, 15 Innovation Way Newark, Delaware 19711.
Carina SihlbomProteomics Core Facility, University of Gothenburg, Medicinaregatan 1G SE 41390 Gothenburg, Sweden.
Barbara AdamczykDepartment of Medical Biochemistry and Cell Biology, University of Gothenburg, Institute of Biomedicine, Sahlgrenska Academy, Medicinaregatan 9A, Box 440, 405 30, Gothenburg, Sweden.
Chunsheng JinDepartment of Medical Biochemistry and Cell Biology, University of Gothenburg, Institute of Biomedicine, Sahlgrenska Academy, Medicinaregatan 9A, Box 440, 405 30, Gothenburg, Sweden.
Niclas G KarlssonDepartment of Medical Biochemistry and Cell Biology, University of Gothenburg, Institute of Biomedicine, Sahlgrenska Academy, Medicinaregatan 9A, Box 440, 405 30, Gothenburg, Sweden.
Jessica ÖrnrosDepartment of Medical Biochemistry and Cell Biology, University of Gothenburg, Institute of Biomedicine, Sahlgrenska Academy, Medicinaregatan 9A, Box 440, 405 30, Gothenburg, Sweden.
Göran LarsonDepartment of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy at the University of Gothenburg, Bruna Straket 16, 41345 Gothenburg, Sweden.
Jonas NilssonDepartment of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy at the University of Gothenburg, Bruna Straket 16, 41345 Gothenburg, Sweden.
Bernd MeyerDepartment of Chemistry, University of Hamburg, Martin Luther King Pl. 6 20146 Hamburg, Germany.
Alena WiegandtDepartment of Chemistry, University of Hamburg, Martin Luther King Pl. 6 20146 Hamburg, Germany.
Emy KomatsuDepartment of Chemistry, University of Manitoba, 144 Dysart Road, Winnipeg, Manitoba, Canada R3T 2N2.
Helene PerreaultDepartment of Chemistry, University of Manitoba, 144 Dysart Road, Winnipeg, Manitoba, Canada R3T 2N2.
Edward D BodnarDepartment of Chemistry, University of Manitoba, 144 Dysart Road, Winnipeg, Manitoba, Canada R3T 2N2; Agilent Technologies, Inc., 5301 Stevens Creek Blvd Santa Clara, California 95051.
Nassur SaidLaboratory of Mass Spectrometry of Interactions and Systems, University of Strasbourg, UMR Unistra-CNRS 7140, France.
Yannis-Nicolas FrancoisLaboratory of Mass Spectrometry of Interactions and Systems, University of Strasbourg, UMR Unistra-CNRS 7140, France.
Emmanuelle Leize-WagnerLaboratory of Mass Spectrometry of Interactions and Systems, University of Strasbourg, UMR Unistra-CNRS 7140, France.
Sandra MaierNatural and Medical Sciences Institute, University of Tübingen, Markwiesenstraβe 55, 72770 Reutlingen, Germany.
Anne ZeckNatural and Medical Sciences Institute, University of Tübingen, Markwiesenstraβe 55, 72770 Reutlingen, Germany.
Albert J R HeckBijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Yang YangBijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Rob HaselbergDivision of Bioanalytical Chemistry, Amsterdam Institute for Molecules, Medicines and Systems, Vrije Universiteit Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
Ying Qing YuDepartment of Chemistry, Waters Corporation, 34 Maple Street Milford, Massachusetts 01757.
William AlleyDepartment of Chemistry, Waters Corporation, 34 Maple Street Milford, Massachusetts 01757.
Joseph W LeoneZoetis, 333 Portage St. Kalamazoo, Michigan 49007.
Hua YuanZoetis, 333 Portage St. Kalamazoo, Michigan 49007.
Stephen E SteinMass Spectrometry Data Center, Biomolecular Measurement Division, Material Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive Gaithersburg, Maryland 20899.

Funding

The incorporation of human milk oligosaccharides in brain glycoconjugatesR01GM049077 · NIGMS · UNIVERSITY OF CALIFORNIA DAVIS · PI LEBRILLA, CARLITO B · 1999 to 2024
$6.3M
Glycoprotein biomarkers for the early detection of aggressive prostate cancerU01CA152813 · NCI · JOHNS HOPKINS UNIVERSITY · PI ZHANG, HUI · 2010 to 2021
$6.2M
The Comprehensive Proteome Characterization Center at Johns Hopkins: High Precision Discovery and Confirmation of Genoproteomic TargetsU24CA210985 · NCI · JOHNS HOPKINS UNIVERSITY · PI CHAN, DANIEL WANYUI, ZHANG, HUI · 2016 to 2020
$5.3M
Sensitive and Quantitative MS-bases Glycomic Mapping PlatformR01GM112490 · NIGMS · TEXAS TECH UNIVERSITY · PI MECHREF, YEHIA · 2014 to 2024
$3.2M
Quantitative Characterization of Glycopeptide IsomersR01GM130091 · NIGMS · TEXAS TECH UNIVERSITY · PI Yehia Mechref · 2019 to 2026
$2.5M
Orbitrap Fusion Tribrid Mass SpectrometerS10OD018530 · OD · UNIVERSITY OF GEORGIA · PI AZADI, PARASTOO · 2014 to 2014
$750k
Establishment and Application of a Library of Site-Specific Glycosylation PatternK01DK101632 · NIDDK · BOSTON CHILDREN'S HOSPITAL · PI FROEHLICH, JOHN WILLIAM · 2014 to 2018
$611k
NCI NIH HHS U01 CA152813NCI NIH HHS U24 CA210985NIDDK NIH HHS K01 DK101632NIGMS NIH HHS R01 GM049077NIGMS NIH HHS R01 GM112490NIGMS NIH HHS R01 GM130091NIH HHS S10 OD018530
6 · The paper itself

Abstract

Glycosylation is a topic of intense current interest in the development of biopharmaceuticals because it is related to drug safety and efficacy. This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material. Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions. The principal objective of this study was to report and compare results for the full range of analytical methods presently used in the glycosylation analysis of mAbs. Therefore, participation was unrestricted, with laboratories choosing their own measurement techniques. Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification. Consequently, the diversity of results was enormous, with the number of glycan compositions identified by each laboratory ranging from 4 to 48. In total, one hundred sixteen glycan compositions were reported, of which 57 compositions could be assigned consensus abundance values. These consensus medians provide community-derived values for NISTmAb PS. Agreement with the consensus medians did not depend on the specific method or laboratory type. The study provides a view of the current state-of-the-art for biologic glycosylation measurement and suggests a clear need for harmonization of glycosylation analysis methods.

Indexed as

Biological ProductsAntibodies, MonoclonalBiopharmaceuticsGlycomicsGlycopeptidesGlycosylationHumansLaboratoriesPolysaccharidesProtein Processing, Post-TranslationalProteomicsAntibodies, MonoclonalBiological ProductsGlycopeptidesPolysaccharidesfluorescenceglycanGlycomicsglycopeptideglycoproteinsglycosylationinterlaboratory studymass spectrometryNISTmAbreference antibody

Identifiers

PMID31591262
PMCPMC6944243

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