ArticlePloS one2019
Agrobacterium tumefaciens-mediated transformation of a hevein-like gene into asparagus leads to stem wilt resistance.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 10 citations in OpenAlex.
- Duckweeds: Model organisms for research on plant sterols and steroids.Plant biology (Stuttgart, Germany) · 2026Review
- Conquering Limitations: Exploring the Factors that Drive Successful Agrobacterium-Mediated Genetic Transformation of Recalcitrant Plant Species.Molecular biotechnology · 2025Review
- Peptides from plants and microorganisms: a defense strategy for biotic and abiotic stresses.Frontiers in plant science · 2025Review
- Genome-wide identification and functional validation of sterol C-22 desaturases and C-24 methyltransferases inFrontiers in plant science · 2025Article
- Transcriptome-Wide Identification and Expression Analysis of Genes Encoding Defense-Related Peptides ofJournal of fungi (Basel, Switzerland) · 2024Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
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Abstract
Asparagus stem wilt, is a significant and devastating disease, typically leading to extensive economic losses in the asparagus industry. To obtain transgenic plants resistant to stem wilt, the hevein-like gene, providing broad spectrum bacterial resistance was inserted into the asparagus genome through Agrobacterium tumefaciens-mediated transformation. The optimal genetic transformation system for asparagus was as follows: pre-culture of embryos for 2 days, inoculation using a bacterial titre of OD600 = 0.6, infection time 10 min and co-culturing for 4 days using an Acetosyringone concentration of 200 μmol/L. Highest transformation frequencies reached 21% and ten transgenic asparagus seedlings carrying the hevein-like gene were identified by polymerase chain reaction. Moreover, integration of the hevein-like gene in the T1 generation of transgenic plants was confirmed by southern blot hybridization. Analysis showed that resistance to stem wilt was enhanced significantly in the transgenic plants, in comparison to non- transgenic plants. The results provide additional data for genetic improvement and are of importance for the development of new disease-resistant asparagus varieties.
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