ArticleJournal of tissue engineering
Rapid porcine corneal decellularization through the use of sodium N-lauroyl glutamate and supernuclease.
Article in Journal of tissue engineering. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Decellularized corneal-based 3D scaffolds: methods decellularization, characterization, mechanical properties, and species source.Regenerative therapy · 2026Review
- Artificial and Bioengineered Therapeutic Options for Corneal Endothelial Disease.Bioengineering (Basel, Switzerland) · 2025Review
- Development of stromal corneal grafts using a novel decellularization method with sodium cocoyl glutamate onAnnals of translational medicine · 2025Article
- Advancements in bioengineering for descemet membrane endothelial keratoplasty (DMEK).NPJ Regenerative medicine · 2025Review
- A pilot study for decellularizing porcine cornea for future use in corneal regeneration.PloS one · 2025Article
- Preparation, physico-biochemical characterization, and proteomic analysis of highly transparent corneal extracellular matrices for lamellar keratoplasty and tissue-engineered cornea construction.Materials today. Bio · 2024Article
- Sodium Dodecyl Sulfate Analogs as a Potential Molecular Biology Reagent.Current issues in molecular biology · 2024Review
- Chemical, Physical, and Biological Corneal Decellularization Methods: A Review of Literature.Journal of ophthalmology · 2024Review
- Preclinical evaluation of the safety and effectiveness of a new bioartificial cornea.Bioactive materials · 2023Article
- Lamellar Keratoplasty Using Acellular Bioengineering Cornea (BioCorneaVetAnimals : an open access journal from MDPI · 2022Article
- Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering.Bioactive materials · 2022Review
- Smart/stimuli-responsive hydrogels: Cutting-edge platforms for tissue engineering and other biomedical applications.Materials today. Bio · 2022Review
- Asymmetry in Drug Permeability through the Cornea.Pharmaceutics · 2021Article
- A decellularized human corneal scaffold for anterior corneal surface reconstruction.Scientific reports · 2021Article
- Comparison of porcine corneal decellularization methods and importance of preserving corneal limbus through decellularization.PloS one · 2021Article
- Engineering a Corneal Stromal Equivalent Using a Novel Multilayered Fabrication Assembly Technique.Tissue engineering. Part A · 2020Article
- Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds.International journal of molecular sciences · 2020Review
- Decellularization for the retention of tissue niches.Journal of tissue engineeringReview
- Post-decellularization techniques ameliorate cartilage decellularization process for tissue engineering applications.Journal of tissue engineeringReview
- Biomaterials for corneal endothelial cell culture and tissue engineering.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Corneal decellularization represents a promising alternative source of human donor with global shortage. Multiple methods have been developed for the preparation of decellularized porcine corneal stroma. However, most strategies relied on long-time treatment to facilitate the entry of detergents or nucleases, which may cause irreversible ultrastructural damage. Here, we developed a rapid decellularization method for porcine corneal stroma through the combined mild detergent sodium N-lauroyl glutamate (SLG) and supernuclease. Compared with traditional methods, the novel decellularization method allowed the efficient removal of xenoantigen DNA within 3 h, while retaining the ultrastructure, transparency, and mechanical properties of porcine corneas. When transplanted in rabbit model for 1 month, the decellularized porcine corneal grafts presented favorable transparency and biocompatibility without immune rejection. Therefore, the combined use of detergent SLG and supernuclease may serve as a promising method for the clinical use of decellularized porcine cornea.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.