ArticleChemical science2019
Structure and effective charge characterization of proteins by a mobility capillary electrophoresis based method.
Article in Chemical science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Advancement of microchip electrophoresis coupled with capacitively coupled contactless conductivity detection-from design to application (2018-2024).Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Review
- Recent advances (2019-2021) of capillary electrophoresis-mass spectrometry for multilevel proteomics.Mass spectrometry reviews · 2023Review
- Fluorescent bioinspired albumin/polydopamine nanoparticles and their interactions withBeilstein journal of nanotechnology · 2023Article
- Recent (2018-2020) development in capillary electrophoresis.Analytical and bioanalytical chemistry · 2022Review
- Measuring how two proteins affect each other's net charge in a crowded environment.Protein science : a publication of the Protein Society · 2021Article
- Toward Nanopore Electrospray Mass Spectrometry: Nanopore Effects in the Analysis of Bacteria.ACS central science · 2020Article
- Rapid 3-dimensional shape determination of globular proteins by mobility capillary electrophoresis and native mass spectrometry.Chemical science · 2020Article
- Predicting Electrophoretic Mobility of Proteoforms for Large-Scale Top-Down Proteomics.Analytical chemistry · 2020Article
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Authors and funding
5 authors.
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Abstract
Measuring the conformations and effective charges of proteins in solution is critical for investigating protein bioactivity, but their rapid analysis remains a challenging problem. Here we report a mobility capillary electrophoresis (MCE) based method for the rapid analysis of protein stereo-structures and effective charges in different solution environments. With the capability of mixture separation, MCE measures the hydrodynamic radius of a protein through Taylor dispersion analysis and its effective charge through ion mobility analysis. The experimental results acquired from MCE are then utilized to restrain molecular dynamics simulations, so that the most probable conformation of that protein can be obtained. As proof-of-concept demonstrations, the charge states and structures of five proteins were analyzed under close to native environments. The conformation transitions and charge state variations of bovine serum albumin and lysozyme under different pH conditions were also investigated. This method is promising for high-throughput protein analysis, which could potentially be coupled with mass spectrometry for investigating protein stereo-structures and functions in top-down proteomics.
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