ArticleBiophysical journal2019
Second Harmonic Generation Spectroscopy of Membrane Probe Dynamics in Gram-Positive Bacteria.
Article in Biophysical journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Monitoring Molecular Interactions with Cell Membranes Using Time-Dependent Second Harmonic Generation Microscopy.Biochemistry · 2025Article
- Quantifying bacterial efflux within subcellular domains ofApplied and environmental microbiology · 2024Article
- Second harmonic scattering investigation of bacterial efflux induced by the antibiotic tetracycline.The Journal of chemical physics · 2024Article
- Miltefosine impacts small molecule transport in Gram-positive bacteria.RSC chemical biology · 2024Article
- Probing bacterial membranes with polarization-resolved second harmonic scattering.Proceedings of SPIE--the International Society for Optical Engineering · 2024Article
- Observing mechanosensitive channels in action in living bacteria.Biophysical reports · 2024Article
- Facilitating flip-flop: Structural tuning of molecule-membrane interactions in living bacteria.Biophysical journal · 2023Article
- Spatial and temporal localization of cell wall associated pili in Enterococcus faecalis.Molecular microbiology · 2023Article
- Monitoring membranes: The exploration of biological bilayers with second harmonic generation.Chemical physics reviews · 2022Review
- Combined Second Harmonic Generation and Fluorescence Analyses of the Structures and Dynamics of Molecules on Lipids Using Dual-Probes: A Review.Molecules (Basel, Switzerland) · 2022Review
- Phosphate Ions Alter the Binding of Daptomycin to Living Bacterial Cell Surfaces.ACS infectious diseases · 2021Article
- Determination of bacterial surface charge density via saturation of adsorbed ions.Biophysical journal · 2021Article
- On the Wireless Microwave Sensing of Bacterial Membrane Potential in Microfluidic-Actuated Platforms.Sensors (Basel, Switzerland) · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Bacterial membranes are complex mixtures with dispersity that is dynamic over scales of both space and time. To capture adsorption onto and transport within these mixtures, we conduct simultaneous second harmonic generation (SHG) and two-photon fluorescence measurements on two different gram-positive bacterial species as the cells uptake membrane-specific probe molecules. Our results show that SHG not only can monitor the movement of small molecules across membrane leaflets but also is sensitive to higher-level ordering of the molecules within the membrane. Further, we show that the membranes of Staphylococcus aureus remain more dynamic after longer times at room temperature in comparison to Enterococcus faecalis. Our findings provide insight into the variability of activities seen between structurally similar molecules in gram-positive bacteria while also demonstrating the power of SHG to examine these dynamics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.