ArticleScientific reports2019
Multiparametric radiobiological assays show that variation of X-ray energy strongly impacts relative biological effectiveness: comparison between 220 kV and 4 MV.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Comparison of In Vitro and Clinical Alpha/Beta Estimates With Potential Impact of Tumor Heterogeneity.Advances in radiation oncology · 2026Article
- Microdosimetry calculations in situ for clinically relevant photon sources and their correlation with the early DNA damage response.Medical physics · 2025Article
- In vitro measurement of proton RBE: A multi-centric comparison using a harmonized setup.Clinical and translational radiation oncology · 2025Article
- Relative biological effectiveness of low-energy X-rays (25 kV) in mutant p53 cancer cells.Radiation and environmental biophysics · 2023Article
- A scoping review of small animal image-guided radiotherapy research: Advances, impact and future opportunities in translational radiobiology.Clinical and translational radiation oncology · 2022Article
- Deep models of integrated multiscale molecular data decipher the endothelial cell response to ionizing radiation.iScience · 2022Article
- Gene expression changes and DNA damage after ex vivo exposure of peripheral blood cells to various CT photon spectra.Scientific reports · 2021Article
- Functionalized Scintillating Nanotubes for Simultaneous Radio- and Photodynamic Therapy of Cancer.ACS applied materials & interfaces · 2021Article
- Review of Experimental Studies to Improve Radiotherapy Response in Bladder Cancer: Comments and Perspectives.Cancers · 2020Review
- Variation of 4 MV X-ray dose rate strongly impacts biological response both in vitro and in vivo.Scientific reports · 2020Article
- Effects of Photons Irradiation onFrontiers in neuroscience · 2020Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Based on classic clonogenic assay, it is accepted by the scientific community that, whatever the energy, the relative biological effectiveness of X-rays is equal to 1. However, although X-ray beams are widely used in diagnosis, interventional medicine and radiotherapy, comparisons of their energies are scarce. We therefore assessed in vitro the effects of low- and high-energy X-rays using Human umbilical vein endothelial cells (HUVECs) by performing clonogenic assay, measuring viability/mortality, counting γ-H2AX foci, studying cell proliferation and cellular senescence by flow cytometry and by performing gene analysis on custom arrays. Taken together, excepted for γ-H2AX foci counts, these experiments systematically show more adverse effects of high energy X-rays, while the relative biological effectiveness of photons is around 1, whatever the quality of the X-ray beam. These results strongly suggest that multiparametric analysis should be considered in support of clonogenic assay.
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