Evidence map›Paper›PMID 31584728›Full record

ArticleMicrocirculation (New York, N.Y. : 1994)2020

Lymphatic-to-blood vessel transition in adult microvascular networks: A discovery made possible by a top-down approach to biomimetic model development.

Mohammad S Azimi, Jessica M Motherwell, Nicholas A Hodges, Garret R Rittenhouse, Dima Majbour, Stacey L Porvasnik, Christine E Schmidt, Walter L Murfee

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microcirculation (New York, N.Y. : 1994), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
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  3. Review
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  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mohammad S AzimiDepartment of Biomedical Engineering, Tulane University, New Orleans, LA, USA.
Jessica M MotherwellDepartment of Biomedical Engineering, Tulane University, New Orleans, LA, USA.
Nicholas A HodgesDepartment of Biomedical Engineering, Tulane University, New Orleans, LA, USA.
Garret R RittenhouseJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Dima MajbourJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Stacey L PorvasnikJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Christine E SchmidtJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.ORCID 0000-0002-5865-6016
Walter L MurfeeJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.ORCID 0000-0002-8247-4722

Funding

Angiogenesis Model for Aging ResearchR01AG049821 · NIA · UNIVERSITY OF FLORIDA · PI MURFEE, WALTER L · 2016 to 2019
$1.0M
NIA NIH HHS R01 AG049821
6 · The paper itself

Abstract

objectiveEmerging areas of vascular biology focus on lymphatic/blood vessel mispatterning and the regulation of endothelial cell identity. However, a fundamental question remains unanswered: Can lymphatic vessels become blood vessels in adult tissues? Leveraging a novel tissue culture model, the objective of this study was to track lymphatic endothelial cell fate over the time course of adult microvascular network remodeling.

methodsCultured adult Wistar rat mesenteric tissues were labeled with BSI-lectin and time-lapse images were captured over five days of serum-stimulated remodeling. Additionally, rat mesenteric tissues on day 0 and day 3 and 5 post-culture were labeled for PECAM + LYVE-1 or PECAM + podoplanin.

resultsCultured networks were characterized by increases in blood capillary sprouting, lymphatic sprouting, and the number of lymphatic/blood vessel connections. Comparison of images from the same network regions identified incorporation of lymphatic vessels into blood vessels. Mosaic lymphatic/blood vessels contained lymphatic marker positive and negative endothelial cells.

conclusionsOur results reveal the ability for lymphatic vessels to transition into blood vessels in adult microvascular networks and discover a new paradigm for investigating lymphatic/blood endothelial cell dynamics during microvascular remodeling.

Indexed as

Models, CardiovascularVascular RemodelingAnimalsCapillariesEndothelial CellsLymphatic VesselsMaleRatsRats, Wistarangiogenesisendothelial celllymphangiogenesislymphaticmicrocirculation

Identifiers

PMID31584728
PMCPMC7033030

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.