Evidence map›Paper›PMID 31584013›Full record

ArticleActa crystallographica. Section F, Structural biology communications2019

Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: expression, purification and crystallization.

Jasper Marc G Bondoc, Hiten J Gutka, Mashal M Almutairi, Ryan Patwell, Maxwell W Rutter, Nina M Wolf, Ram Samudrala, Shahila Mehboob, Farahnaz Movahedzadeh

Erratum issuedOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Jasper Marc G BondocInstitute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA.
Hiten J GutkaInstitute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA.
Mashal M AlmutairiInstitute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA.
Ryan PatwellInstitute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA.
Maxwell W RutterInstitute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA.
Nina M WolfInstitute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA.
Ram SamudralaDepartment of Biomedical Informatics, Jacobs School of Medicine and Biomedical Sciences, State University of New York (SUNY), University at Buffalo, 77 Goodell Street, Buffalo, NY 14203, USA.
Shahila MehboobNeugenica LLC, 2242 West Harrison Street #201, Chicago, IL 60612, USA.
Farahnaz MovahedzadehInstitute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA.
University of Illinois Chicago · USKing Saud University · SAUniversity at Buffalo, State University of New York · US

Funding

NOVEL PARADIGMS FOR DRUG DISCOVERY: COMPUTATIONAL MULTITARGET SCREENINGDP1LM011509 · NLM · UNIVERSITY OF WASHINGTON · PI SAMUDRALA, RAM · 2012 to 2014
$2.4M
NOVEL PARADIGMS FOR DRUG DISCOVERY: COMPUTATIONAL MULTITARGET SCREENINGDP1OD006779 · OD · UNIVERSITY OF WASHINGTON · PI SAMUDRALA, RAM · 2010 to 2011
$1.7M
American Lung Association RG-82534-NNIH HHS DP1 OD006779NLM NIH HHS DP1 LM011509
6 · The paper itself

Abstract

Acyl carrier proteins (ACPs) are important components in fatty-acid biosynthesis in prokaryotes. Rv0100 is predicted to be an essential ACP in Mycobacterium tuberculosis, the pathogen that is the causative agent of tuberculosis, and therefore has the potential to be a novel antituberculosis drug target. Here, the successful cloning and purification of Rv0100 using Mycobacterium smegmatis as a host is reported. Crystals of the purified protein were obtained that diffracted to a resolution of 1.9 Å. Overall, this work lays the foundation for the future pursuit of drug discovery and development against this potentially novel drug target.

Indexed as

CrystallizationAcyl Carrier ProteinBacterial ProteinsCrystallography, X-RayMycobacterium smegmatisMycobacterium tuberculosisProtein ConformationRecombinant ProteinsAcyl Carrier ProteinBacterial ProteinsRecombinant Proteinsacyl carrier proteinMycobacterium smegmatisMycobacterium tuberculosisRv0100

Identifiers

PMID31584013
PMCPMC6777135
OpenAlexW4205945032

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.