ArticleMolecular biology reports2020
Stable reference genes for expression studies in breast muscle of normal and white striping-affected chickens.
Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Involvement of Fibro-Adipogenic Progenitors and Cellular Communication Network Family Signaling in the Impaired Muscle Regeneration of Wooden Breast.The journal of poultry science · 2026Article
- Screening of reliable reference genes for the normalization of RT-qPCR in chicken liver tissues and LMH cells.Scientific reports · 2024Article
- Article
- Identification of appropriate housekeeping genes for gene expression studies in human renal cell carcinoma under hypoxic conditions.Molecular biology reports · 2022Article
- Reference gene selection for expression studies in the reproductive axis tissues of Magang geese at different reproductive stages under light treatment.Scientific reports · 2021Article
- Differential Expression of Myogenic and Calcium Signaling-Related Genes in Broilers Affected With White Striping.Frontiers in physiology · 2021Article
- Screening of Reference Genes for RT-qPCR in Chicken Adipose Tissue and Adipocytes.Frontiers in physiology · 2021Article
- Reference genes for proximal femoral epiphysiolysis expression studies in broilers cartilage.PloS one · 2020Article
- Selection and Validation of the Optimal Panel of Reference Genes for RT-qPCR Analysis in the Developing Rat Cartilage.Frontiers in genetics · 2020Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The normalization with proper reference genes is a crucial step to obtain accurate mRNA expression levels in quantitative PCR (qPCR) studies. Therefore, in this study, 10 reference candidate genes were evaluated to determine their stability in normal pectoralis major muscle of broilers and those counterparts affected with White Striping (WS) myopathy at 42 days age. Four different tools were used for ranking the most stable genes: GeNorm, NormFinder, BestKeeper and Comparative Ct (ΔCt), and a general ranking was performed using the RankAggreg tool to select the best reference genes among all tools. From the 10 genes evaluated in the breast muscle of broilers, 8 were amplified. Most of the algorithms/tools indicated the same two genes, RPL30 and RPL5, as the most stable in the broilers breast muscle. In addition, there was agreement among the tools for the least stable genes: MRPS27, GAPDH and RPLP1 in the broilers breast muscle. Therefore, it is interesting to note that even with different tools for evaluating gene expression, there was consensus on the most and least stable genes. These results indicate that the Ribosomal protein L30 (RPL30) and Ribosomal protein L5 (RPL5) can be recommended for accurate normalization in qPCR studies with chicken pectoralis major muscle affected with White Striping and other myopathies.
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