Evidence map›Paper›PMID 31583241›Full record

ArticleJournal of advanced veterinary and animal research2019

Development and validation of BLRI Mastitis Test Kit at Bangladesh Livestock Research Institute Regional Station, Sirajganj.

Md Humayun Kabir, Md Ershaduzzaman, K H M Nazmul Hussain Nazir, Mohammad Sirajul Islam, Razia Khatun, Md Shahjahan Ali Sarker, Md Abu Yousuf, Yousuf Ali, Nathu Ram Sarkar, Md Giasuddin

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Article in Journal of advanced veterinary and animal research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Md Humayun KabirBangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh.
Md ErshaduzzamanBangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh.
K H M Nazmul Hussain NazirDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Mohammad Sirajul IslamBangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh.
Razia KhatunBangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh.
Md Shahjahan Ali SarkerDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Md Abu YousufBangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh.
Yousuf AliBangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh.
Nathu Ram SarkarBangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh.
Md GiasuddinBangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe objective of this study was to develop a low-cost kit for the detection of subclinical mastitis (SCM) and to check its validity, reproducibility, and efficacy at the field level. MATERIALS AND

methodsA total of 550 quarter milk samples from crossbred dairy cows were collected, of which 400 milk samples were used to validate the newly developed BLRI mastitis test (BMT) kit to justify its efficacy as an individual test kit in detecting SCM based on somatic cell count (SCC) by direct microscopic count (DMC). The efficacy of the newly developed BMT was compared with the California Mastitis Test (CMT) kit. Another 150 milk samples were subjected to SCC determined by DMC and DCC (De Laval cell counter®) categorized by CMT and BMT scores.

resultsA SCM test kit, namely, BMT kit was successfully developed in this study. The percentage accuracy of CMT and BMT were 76.75% and 75.75%; sensitivity 69.36% and 67.56%; specificity 85.95% and 85.85%; positive predictive value 86.03% and 85.71%; negative predictive value 69.23% and 68%, respectively. A p value of 0.001 was found for both CMT and BMT. However, CMT and BMT had no significant difference in sensitivity (p = 0.778). Average SCCs (cells/ml) determined by DCC and DMC, respectively, were mostly corresponded to the SCC ranges of both CMT and BMT scores.

conclusionThe newly developed BMT kit is an independent, cheap, farmer-friendly, first country made, and reliable SCM diagnostic test kit that can be used at field condition.

Indexed as

AccuracyBMTCMTefficacymastitisvalidity

Identifiers

PMID31583241
PMCPMC6760501

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