ArticleBiotechnology for biofuels2019
Fusion transcription factors for strong, constitutive expression of cellulases and xylanases in
Article in Biotechnology for biofuels, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 103 citations in OpenAlex.
- Research Advances on the Functions of Transcriptional Regulators in Filamentous Fungi.Microorganisms · 2026Review
- In Vivo Characterization of theJournal of fungi (Basel, Switzerland) · 2026Article
- Robust Production of Cellulases to Develop a Low-Cost Sugar Platform.Advances in biochemical engineering/biotechnology · 2026Review
- Providing a toolbox for genomic engineering ofMicrobiology spectrum · 2025Article
- Improvement of β-Xylosidase and Endoxylanase Activities in Talaromyces amestolkiae by Genetic Manipulation of the Transcriptional Activator XlnR.Microbial biotechnology · 2025Article
- Discovery of the antifungal compound ilicicolin K through genetic activation of the ilicicolin biosynthetic pathway in Trichoderma reesei.Biotechnology for biofuels and bioproducts · 2025Article
- Life on the rocks: unexpected enzyme activity of the extremophilic black fungusFrontiers in bioengineering and biotechnology · 2025Article
- Cumulative expression of heterologous XlnR regulatory modules and AraRBiotechnology for biofuels and bioproducts · 2024Article
- Investigating the cellular functions of β-Glucosidases for synthesis of lignocellulose-degrading enzymes inEngineering microbiology · 2023Article
- Structure-guided engineering of transcriptional activator XYR1 for inducer-free production of lignocellulolytic enzymes inSynthetic and systems biotechnology · 2023Article
- The Ras small GTPase RSR1 regulates cellulase production in Trichoderma reesei.Biotechnology for biofuels and bioproducts · 2023Article
- Intracellular Sugar Transporters Facilitate Cellulase Synthesis inBiomolecules · 2023Article
- Role of the Nitrogen Metabolism Regulator TAM1 in Regulation of Cellulase Gene Expression in Trichoderma reesei.Applied and environmental microbiology · 2023Article
- Botrytis cinerea Transcription Factor BcXyr1 Regulates (Hemi-)Cellulase Production and Fungal Virulence.mSystems · 2022Article
- Discovery of ER-localized sugar transporters for cellulase production with lac1 being essential.Biotechnology for biofuels and bioproducts · 2022Article
- Inducer-free cellulase production system based on the constitutive expression of mutated XYR1 and ACE3 in the industrial fungus Trichoderma reesei.Scientific reports · 2022Article
- Factors regulating cellulolytic gene expression in filamentous fungi: an overview.Microbial cell factories · 2022Review
- Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi.Frontiers in bioengineering and biotechnology · 2022Review
- Modular Synthetic Biology Toolkit for Filamentous Fungi.ACS synthetic biology · 2021Article
- Glutamine involvement in nitrogen regulation of cellulase production in fungi.Biotechnology for biofuels · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe filamentous ascomycete
resultsHere, we constructed a Xyr1-deficient strain expressing a non-functional truncated version of Xyr1. This strain was successfully used as platform strain for overexpression of Xyr1, which enhanced the cellulase and xylanase production rates under inducing conditions, with the exception of lactose-there the cellulase production was severely reduced. Further, we introduced fusion transcription factors consisting of the DNA-binding domain of Xyr1 and the transactivation domain of either Ypr1 or Ypr2 (regulators of the sorbicillinoid biosynthesis gene cluster). The fusion of Xyr1 and Ypr2 yielded a moderately transactivating transcription factor, whereas the fusion of Xyr1 and Ypr1 yielded a highly transactivating transcription factor that induced xylanases and cellulases nearly carbon source independently. Especially, high production levels of xylanases were achieved on glycerol.
conclusionDuring this study, we constructed a Xyr1-deficient strain that can be fully reconstituted, which makes it an ideal platform strain for Xyr1-related studies. The mere overexpression of Xyr1 turned out not to be a successful strategy for overall enhancement of the enzyme production rates. We gained new insights into the regulatory properties of transcription factors by constructing respective fusion proteins. The Xyr1-Ypr1-fusion transcription factor could induce xylanase production rates on glycerol to outstanding extents, and hence could be deployed in the future to utilize crude glycerol, the main co-product of the biodiesel production process.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.