ArticleNature structural & molecular biology2019
FACT mediates cohesin function on chromatin.
Article in Nature structural & molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- FACT depletion demonstrates a role for nucleosome organization in TAD formation.Molecular systems biology · 2026Article
- Structural Maintenance of Chromosomes Complexes.Methods in molecular biology (Clifton, N.J.) · 2025Review
- CRISPR screens in sister chromatid cohesion defective cells reveal PAXIP1-PAGR1 as regulator of chromatin association of cohesin.Nucleic acids research · 2023Article
- ISW1a modulates cohesin distribution in centromeric and pericentromeric regions.Nucleic acids research · 2023Article
- Loops and the activity of loop extrusion factors constrain chromatin dynamics.Molecular biology of the cell · 2023Article
- Article
- Depletion or cleavage of cohesin during anaphase differentially affects chromatin structure and segregation.eLife · 2022Article
- Karyotype engineering reveals spatio-temporal control of replication firing and gene contacts.Cell genomics · 2022Article
- Mediator recruits the cohesin loader Scc2 to RNA Pol II-transcribed genes and promotes sister chromatid cohesion.Current biology : CB · 2022Article
- Cohesin-dependent chromosome loop extrusion is limited by transcription and stalled replication forks.Science advances · 2022Article
- Article
- Review
- The Interplay of Cohesin and the Replisome at Processive and Stressed DNA Replication Forks.Cells · 2021Review
- A compendium of chromatin contact maps reflecting regulation by chromatin remodelers in budding yeast.Nature communications · 2021Article
- The histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states.Cell reports · 2021Article
- Cohesin regulates homology search during recombinational DNA repair.Nature cell biology · 2021Article
- Article
- Review
- Sir3 mediates long-range chromosome interactions in budding yeast.Genome research · 2021Article
- The role of FACT in managing chromatin: disruption, assembly, or repair?Nucleic acids research · 2020Review
Corrections and comments
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Authors and funding
16 authors at 3 institutions in 3 countries.
Funding
Abstract
Cohesin is a regulator of genome architecture with roles in sister chromatid cohesion and chromosome compaction. The recruitment and mobility of cohesin complexes on DNA is restricted by nucleosomes. Here, we show that the role of cohesin in chromosome organization requires the histone chaperone FACT ('facilitates chromatin transcription') in Saccharomyces cerevisiae. We find that FACT interacts directly with cohesin, and is dynamically required for its localization on chromatin. Depletion of FACT in metaphase cells prevents cohesin accumulation at pericentric regions and causes reduced binding on chromosome arms. Using the Hi-C technique, we show that cohesin-dependent TAD (topological associated domain)-like structures in G1 and metaphase chromosomes are reduced in the absence of FACT. Sister chromatid cohesion is intact in FACT-depleted cells, although chromosome segregation failure is observed. Our data show that FACT contributes to the formation of cohesin-dependent TADs, thus uncovering a new role for this complex in nuclear organization during interphase and mitotic chromosome folding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.