ArticleNature structural & molecular biology2019
Cryo-EM of multiple cage architectures reveals a universal mode of clathrin self-assembly.
Article in Nature structural & molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 87 citations in OpenAlex.
- Interview With Barbara Pearse and the Clathrin Family Tree.Traffic (Copenhagen, Denmark) · 2026Article
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- Microstrain-engineered platinum nanoclathrins for fuel cells.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Clathrins Are Involved in the Endocytosis of Host Cytosol in the Malaria Parasite.Traffic (Copenhagen, Denmark) · 2026Article
- Programmable DNA shell scaffolds for directional membrane budding.Nature communications · 2025Article
- Inhibition of the Clathrin Terminal Domain-Amphiphysin Protein-Protein Interaction. Probing the Pitstop 2 Aromatic Moiety.ChemMedChem · 2025Article
- Flexing protein assemblies.Nature structural & molecular biology · 2025Article
- Local structural flexibility drives oligomorphism in computationally designed protein assemblies.Nature structural & molecular biology · 2025Article
- Interface flexibility controls the nucleation and growth of supramolecular networks.Nature chemistry · 2025Article
- A new statistical metric for robust target detection in cryo-EM using 2D template matching.IUCrJ · 2025Article
- Cryo-electron tomography pipeline for plasma membranes.Nature communications · 2025Article
- Molecular architecture of synaptic vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Subtleties in Clathrin heavy chain binding boxes provide selectivity among adaptor proteins of budding yeast.Nature communications · 2024Article
- Bottom-up construction of chiral metal-peptide assemblies from metal cluster motifs.Nature communications · 2024Article
- Mechanistic divergences of endocytic clathrin-coated vesicle formation in mammals, yeasts and plants.Journal of cell science · 2024Review
- Lipid droplets as substrates for protein phase separation.Biophysical journal · 2024Article
- Mitigating the Blurring Effect of CryoEM Averaging on a Flexible and Highly Symmetric Protein Complex through Sub-Particle Reconstruction.International journal of molecular sciences · 2024Article
- Clathrin coats partially preassemble and subsequently bend during endocytosis.The Journal of cell biology · 2023Article
- Imaging nanoscale axial dynamics at the basal plasma membrane.The international journal of biochemistry & cell biology · 2023Review
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Clathrin forms diverse lattice and cage structures that change size and shape rapidly in response to the needs of eukaryotic cells during clathrin-mediated endocytosis and intracellular trafficking. We present the cryo-EM structure and molecular model of assembled porcine clathrin, providing insights into interactions that stabilize key elements of the clathrin lattice, namely, between adjacent heavy chains, at the light chain-heavy chain interface and within the trimerization domain. Furthermore, we report cryo-EM maps for five different clathrin cage architectures. Fitting structural models to three of these maps shows that their assembly requires only a limited range of triskelion leg conformations, yet inherent flexibility is required to maintain contacts. Analysis of the protein-protein interfaces shows remarkable conservation of contact sites despite architectural variation. These data reveal a universal mode of clathrin assembly that allows variable cage architecture and adaptation of coated vesicle size and shape during clathrin-mediated vesicular trafficking or endocytosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.