ArticleMicroorganisms2019
Targeted High-Throughput Sequencing Identifies Predominantly Fungal Pathogens in Patients with Clinically Infectious, Culture-Negative Endophthalmitis in South India.
Article in Microorganisms, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Acute fungal post-cataract endophthalmitis in the endophthalmitis management study: EMS report 7.BMJ open ophthalmology · 2025Trial
- Understanding the science of fungal endophthalmitis - AIOS 2021 Sengamedu Srinivas Badrinath Endowment Lecture.Indian journal of ophthalmology · 2022Trial
- A case report of ocular infection caused byAME case reports · 2026Article
- International consensuses and guidelines on diagnosing and managing fungal endophthalmitis by the Asia-Pacific Vitreo-retina Society (APVRS), the Academy of the Asia-Pacific Professors of Ophthalmology (AAPPO), and the Asia-Pacific Society of Ocular Inflammation and Infection (APSOII).Eye and vision (London, England) · 2025Review
- Clinical performance of nanopore targeted sequencing for diagnosing endophthalmitis.BMC microbiology · 2025Article
- Development and validation of a nomogram to predict bacterial blood stream infection.European journal of medical research · 2025Article
- The Diagnostic Value of Triggering Receptor Expressed on Myeloid Cells-1 in Post-Traumatic Bacterial Endophthalmitis.Investigative ophthalmology & visual science · 2023Article
- Rapid and precise identification of bloodstream infections using a pre-treatment protocol combined with high-throughput multiplex genetic detection system.BMC infectious diseases · 2022Article
- Update on Current Microbiological Techniques for Pathogen Identification in Infectious Endophthalmitis.International journal of molecular sciences · 2022Review
- The Diagnosis and Treatment of Fungal Endophthalmitis: An Update.Diagnostics (Basel, Switzerland) · 2022Review
- Application of High-Throughput Sequencing Technology in Identifying the Pathogens in Endophthalmitis.Journal of ophthalmology · 2022Review
- Nanopore techniques as a potent tool in the diagnosis and treatment of endophthalmitis: a literature review.International journal of ophthalmology · 2022Review
- Microbiological diagnosis of endophthalmitis using nanopore targeted sequencing.Clinical & experimental ophthalmology · 2021Article
- Fungal biodiversity and conservation mycology in light of new technology, big data, and changing attitudes.Current biology : CB · 2021Review
- Interaction of environmental eukaryotic microorganisms and fungi in the pond-cultured carps: new insights into the potential pathogenic fungi in the freshwater aquaculture.Environmental science and pollution research international · 2021Article
- Microglial Response toJournal of fungi (Basel, Switzerland) · 2020Article
- Article
- Endophthalmitis Management: Stain-Culture, Empirical Treatment, and Beyond.Asia-Pacific journal of ophthalmology (Philadelphia, Pa.)Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
To evaluate the clinical utility of high-throughput sequencing (HTS) approach-based analysis of the bacterial and fungal genome in vitreous fluids from patients clinically diagnosed as endophthalmitis, we subjected 75 vitreous fluids from clinically presumed infectious endophthalmitis patients to high-throughput sequencing (Illumina HiSeq 2500) after DNA extraction and amplification of the 16S rRNA for the detection of bacteria, and ITS 2 region for detection of fungal pathogens. As controls, we included vitreous biopsies from 70 patients diagnosed with other non-infectious retinal disorders. Following the construction of the curated microbial genome database and filtering steps to reduce ambiguousness/contaminants from the environment, the paired reads were analyzed. Our HTS reads revealed in almost all cases the same organism that was grown in culture (bacterial-14/15, fungal 3/3) by conventional microbiological workup. HTS additionally diagnosed the presence of microbes in 42/57 (73.7%) patients who were conventionally negative (fungal pathogens in 36/57, bacterial pathogens in 11/57, including five cases that showed the presence of both bacterial and fungal organisms).
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.