ArticleeLife2019
Endothelial PKA activity regulates angiogenesis by limiting autophagy through phosphorylation of ATG16L1.
Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Vasoactive intestinal peptide reduces ocular hypertension by regulating tight junction of trabecular meshwork through Rab13/PKA signalling complex.Annals of medicine · 2025Article
- Protein kinase A regulates ferroptosis by controlling GPX4 mCell death and differentiation · 2025Article
- Autophagosomes anchor an AKAP11-dependent regulatory checkpoint that shapes neuronal PKA signaling.The EMBO journal · 2025Article
- Regulation of Sleep Amount by CRTC1 via Transcription ofThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- ZDHHC7-mediatedAutophagy · 2024Article
- Article
- Defective BVES-mediated feedback control of cAMP in muscular dystrophy.Nature communications · 2023Article
- ZNF185 prevents stress fiber formation through the inhibition of RhoA in endothelial cells.Communications biology · 2023Article
- Suppression of CCL2 angiocrine function by adrenomedullin promotes tumor growth.The Journal of experimental medicine · 2023Article
- Autophagy regulates neuronal excitability by controlling cAMP/protein kinase A signaling at the synapse.The EMBO journal · 2022Article
- Vascular and Liver Homeostasis in Juvenile Mice Require Endothelial Cyclic AMP-Dependent Protein Kinase A.International journal of molecular sciences · 2022Article
- Targeting tumor innervation: premises, promises, and challenges.Cell death discovery · 2022Review
- The role of autophagy in cardiovascular pathology.Cardiovascular research · 2022Review
- Review
- Identification of kinases activated by multiple pro-angiogenic growth factors.Frontiers in pharmacology · 2022Article
- PKA compartmentalization links cAMP signaling and autophagy.Cell death and differentiation · 2021Article
- Selective autophagy of AKAP11 activates cAMP/PKA to fuel mitochondrial metabolism and tumor cell growth.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Genetic Variants and Functional Analyses of theFrontiers in genetics · 2021Article
- Autophagy in the Regulation of Tissue Differentiation and Homeostasis.Frontiers in cell and developmental biology · 2020Review
- Endothelial PKA activity regulates angiogenesis by limiting autophagy through phosphorylation of ATG16L1.eLife · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 3 institutions in 4 countries.
Funding
Abstract
The cAMP-dependent protein kinase A (PKA) regulates various cellular functions in health and disease. In endothelial cells PKA activity promotes vessel maturation and limits tip cell formation. Here, we used a chemical genetic screen to identify endothelial-specific direct substrates of PKA in human umbilical vein endothelial cells (HUVEC) that may mediate these effects. Amongst several candidates, we identified ATG16L1, a regulator of autophagy, as novel target of PKA. Biochemical validation, mass spectrometry and peptide spot arrays revealed that PKA phosphorylates ATG16L1α at Ser268 and ATG16L1β at Ser269, driving phosphorylation-dependent degradation of ATG16L1 protein. Reducing PKA activity increased ATG16L1 protein levels and endothelial autophagy. Mouse in vivo genetics and pharmacological experiments demonstrated that autophagy inhibition partially rescues vascular hypersprouting caused by PKA deficiency. Together these results indicate that endothelial PKA activity mediates a critical switch from active sprouting to quiescence in part through phosphorylation of ATG16L1, which in turn reduces endothelial autophagy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.