Evidence map›Paper›PMID 31578319›Full record

ArticleInternational journal of oral science2019

Filamentation initiated by Cas2 and its association with the acquisition process in cells.

Lei Wang, Xin Yu, Mengjie Li, Guiqin Sun, Lin Zou, Tiansheng Li, Linlin Hou, Yameng Guo, Danfeng Shen, Di Qu and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of oral science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.2field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Lei WangDepartment of Medical Microbiology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, 131 Dongan Road, Shanghai, China.
Xin YuDepartment of Medical Microbiology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, 131 Dongan Road, Shanghai, China.ORCID http://orcid.org/0000-0001-7713-2106
Mengjie LiDepartment of Medical Microbiology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, 131 Dongan Road, Shanghai, China.
Guiqin SunCollege of Medical Technology, Zhejiang Chinese Medical University, Hangzhou, China.
Lin ZouDepartment of Medical Microbiology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, 131 Dongan Road, Shanghai, China.
Tiansheng LiDepartment of Medical Microbiology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, 131 Dongan Road, Shanghai, China.
Linlin HouDepartment of Medical Microbiology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, 131 Dongan Road, Shanghai, China.
Yameng GuoDepartment of Medical Microbiology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, 131 Dongan Road, Shanghai, China.
Danfeng ShenDepartment of Medical Microbiology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, 131 Dongan Road, Shanghai, China.
Di QuDepartment of Medical Microbiology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, 131 Dongan Road, Shanghai, China.
Xunjia ChengDepartment of Medical Microbiology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, 131 Dongan Road, Shanghai, China.
Li ChenDepartment of Medical Microbiology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, 131 Dongan Road, Shanghai, China. lichen_bk@fudan.edu.cn.ORCID http://orcid.org/0000-0002-7006-4443
Fudan University · CNShanghai Medical College of Fudan University · CNZhejiang Chinese Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cas1-and-Cas2-mediated new spacer acquisition is an essential process for bacterial adaptive immunity. The process is critical for the ecology of the oral microflora and oral health. Although molecular mechanisms for spacer acquisition are known, it has never been established if this process is associated with the morphological changes of bacteria. In this study, we demonstrated a novel Cas2-induced filamentation phenotype in E. coli that was regulated by co-expression of the Cas1 protein. A 30 amino acid motif at the carboxyl terminus of Cas2 is necessary for this function. By imaging analysis, we provided evidence to argue that Cas-induced filamentation is a step coupled with new spacer acquisition during which filaments are characterised by polyploidy with asymmetric cell division. This work may open new opportunities to investigate the adaptive immune response and microbial balance for oral health.

Indexed as

Oral HealthAdaptation, BiologicalAdaptive ImmunityBacterial ProteinsCRISPR-Cas SystemsDNA, BacterialEndonucleasesEscherichia coliEscherichia coli ProteinsHumansImmunityBacterial ProteinsCas2 protein, E coliDNA, BacterialEndonucleasesEscherichia coli Proteins

Identifiers

PMID31578319
PMCPMC6802651
OpenAlexW2978551217

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.