Evidence map›Paper›PMID 31576812›Full record

ArticleAging2019

LncRNAs down-regulate Myh1, Casr, and Mis18a expression in the Substantia Nigra of aged male rats.

Guoliang Zhang, Yunxiao Kang, Xu Feng, Rui Cui, Qiqing Guo, Xiaoming Ji, Yuanxiang Huang, Yannan Ma, Shufeng Liu, Geming Shi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Aging, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Guoliang ZhangDepartment of Neurobiology, Hebei Medical University, Hebei Province, Shijiazhuang, 050017, China.
Yunxiao KangDepartment of Neurobiology, Hebei Medical University, Hebei Province, Shijiazhuang, 050017, China.
Xu FengHebei Laboratory Animal Center, Hebei Medical University, Hebei Province, Shijiazhuang, 050017, China.
Rui CuiDepartment of Human Anatomy, Hebei Medical University, Hebei Province, Shijiazhuang, 050017, China.
Qiqing GuoDepartment of Neurobiology, Hebei Medical University, Hebei Province, Shijiazhuang, 050017, China.
Xiaoming JiDepartment of Neurobiology, Hebei Medical University, Hebei Province, Shijiazhuang, 050017, China.
Yuanxiang HuangGrade 2015 Eight-year Clinical Medicine Program, School of Basic Medical Sciences, Hebei Medical University, Hebei Province, Shijiazhuang, 050017, China.
Yannan MaDepartment of Neurobiology, Hebei Medical University, Hebei Province, Shijiazhuang, 050017, China.
Shufeng LiuHebei Laboratory Animal Center, Hebei Medical University, Hebei Province, Shijiazhuang, 050017, China.
Geming ShiDepartment of Neurobiology, Hebei Medical University, Hebei Province, Shijiazhuang, 050017, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we used high-throughput RNA sequencing to identify mRNAs, long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) that are differentially expressed in the Substantia Nigra (SN) of aged and young rats. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were used to perform functional annotation of mRNAs that were either differentially expressed themselves (DEMs), targeted by differentially expressed lncRNAs (DELs), or the parents of differentially expressed circRNAs (DECs). A total of 112 DEMs, 163 DELs, and 98 DECs were found in the SN of aged rats. The down-regulated lncRNA NONRATT010417.2 targeted the down-regulated mRNA Myh1, while the down-regulated lncRNA NONRATT015586.2 and the up-regulated lncRNAs NONRATT000490.2 and NONRATT007029.2 all targeted the down-regulated mRNAs Casr and Mis18a. Western blots and RT-qPCR revealed that Myh1, Casr, and Mis18a protein and mRNA expression were significantly reduced in aged rats compared to young rats. This study improves our understanding of the transcriptional alterations underlying aging-related changes in the SN and provides a foundation for future studies of associated molecular mechanisms.

Indexed as

AgingAnimalsDown-RegulationGene Expression ProfilingMaleMyosin Heavy ChainsRatsReceptors, Calcium-SensingRNA, CircularRNA, Long NoncodingSubstantia NigraTranscription, Geneticextracellular calcium cation-sensing receptor, ratMyh1 protein, ratMyosin Heavy ChainsReceptors, Calcium-SensingRNA, CircularRNA, Long Noncodingaged male ratscircular RNAslong non-coding RNAsmessenger RNAsSubstantia Nigra

Identifiers

PMID31576812
PMCPMC6814601

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.