Evidence map›Paper›PMID 31576466›Full record

ArticlePediatric surgery international2019

A comparison of exosomes derived from different periods breast milk on protecting against intestinal organoid injury.

Runnan Gao, Rong Zhang, Tian Qian, Xueni Peng, Weijing He, Shan Zheng, Yun Cao, Agostino Pierro, Chun Shen

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Pediatric surgery international, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed
5.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 78 citations in OpenAlex.

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  7. Milk-Derived Extracellular Vesicles and microRNAs: Potential Modulators of Intestinal Homeostasis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Runnan GaoDepartment of Pediatric Surgery, Children's Hospital of Fudan University, 399 Wanyuan RD, Shanghai, 201102, China.
Rong ZhangDepartment of Neonatology, Children's Hospital of Fudan University, Shanghai, China.
Tian QianDepartment of Neonatology, Children's Hospital of Fudan University, Shanghai, China.
Xueni PengDepartment of Pediatric Surgery, Children's Hospital of Fudan University, 399 Wanyuan RD, Shanghai, 201102, China.
Weijing HeDepartment of Pediatric Surgery, Children's Hospital of Fudan University, 399 Wanyuan RD, Shanghai, 201102, China.
Shan ZhengDepartment of Pediatric Surgery, Children's Hospital of Fudan University, 399 Wanyuan RD, Shanghai, 201102, China.
Yun CaoDepartment of Neonatology, Children's Hospital of Fudan University, Shanghai, China.
Agostino PierroDivision of General and Thoracic Surgery, The Hospital for Sick Children, Toronto, ON, Canada.
Chun ShenDepartment of Pediatric Surgery, Children's Hospital of Fudan University, 399 Wanyuan RD, Shanghai, 201102, China. chunshen@fudan.edu.cn.
Children's Hospital of Fudan University · CNHospital for Sick Children · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aim of the studyHuman breast milk reduces the risk and severity of necrotizing enterocolitis (NEC). Exosomes are extracellular vesicles (EVs) found in high concentrations in milk, and they mediate intercellular communication and immune responses. The aim of this study is to compare the protective effects of exosomes that are derived from different time periods of breast milk production against intestinal injury using an ex vivo intestinal organoid model.

methodsColostrum, transitional and mature breast milk samples from healthy lactating mothers were collected. Exosomes were isolated using serial ultracentrifugation and filtration. Exosomes' presence was confirmed using transmission electron microscopy (TEM) and western blot. To form the intestinal organoids, terminal ileum was harvested from neonatal mice pups at postnatal day 9, crypts were isolated and organoids were cultured in matrigel. Organoids were either cultured with exposure to lipopolysaccharide (LPS), or in treatment groups where both LPS and exosomes were added in the culturing medium. Inflammatory markers and organoids viability were evaluated. MAIN

resultsHuman milk-derived exosomes were successfully isolated and characterized. LPS administration reduced the size of intestinal organoids, induced inflammation through increasing TNFα and TLR4 expression, and stimulated intestinal regeneration. Colostrum, transitional and mature human milk-derived exosome treatment all prevented inflammatory injury, while exosomes derived from colostrum were most effective at reducing inflammatory cytokine.

conclusionsHuman breast milk-derived exosomes were able to protect intestine organoids against epithelial injury induced by LPS. Colostrum exosomes offer the best protective effect among the breast-milk derived exosomes. Human milk exosomes can be protective against the development of intestinal injury such as that seen in NEC.

Indexed as

AnimalsColostrumDisease Models, AnimalEnterocolitis, NecrotizingExosomesFemaleHumansIntestinal MucosaLactationMiceMice, Inbred C57BLMilk, HumanOrganoidsExosomesHuman breast milkIntestinal organoidsNecrotizing enterocolitis (NEC)

Identifiers

PMID31576466
OpenAlexW2978951376

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.