Evidence map›Paper›PMID 31576447›Full record

ArticlePlanta2019

Characterization of feruloyl esterases in maize pollen.

Marcia M de O Buanafina, M Fernanda Buanafina, Tatiana Laremore, Erica A Shearer, Howard W Fescemyer

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.2field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Marcia M de O BuanafinaDepartment of Biology, 208 Mueller Laboratory, The Pennsylvania State University, University Park, PA, 16802, USA. mmb26@psu.edu.
M Fernanda BuanafinaDepartment of Biology, 208 Mueller Laboratory, The Pennsylvania State University, University Park, PA, 16802, USA.
Tatiana LaremorePenn State Proteomics and Mass Spectrometry Core Facility, The Pennsylvania State University, University Park, PA, 16802, USA.
Erica A ShearerDepartment of Biology, 208 Mueller Laboratory, The Pennsylvania State University, University Park, PA, 16802, USA.
Howard W FescemyerDepartment of Biology, 208 Mueller Laboratory, The Pennsylvania State University, University Park, PA, 16802, USA.
Pennsylvania State University · US

Funding

Department Of Energy DE-FG02-08ER64701US Department of Agriculture 2009-35318-0513
6 · The paper itself

Abstract

MAIN

conclusionFerulic acid esterases have been identified and partially purified from maize pollen. Results suggest that maize pollen FAEs may play an important role in pollen fertilization. A critical step in maize (Zea mays) seed production involves fertilization of the ovule by pollen, a process that relies on ability of the pollen tube to grow through the highly structured and feruloylated arabinoxylan/cellulose-rich tissue of the silk and stigma. It is known that different cell wall hydrolases are present on the surface of pollen. An important hydrolase reported to date is an endo-xylanase (ZmXYN1). We report presence and characterization of another hydrolase, ferulic acid esterase (FAE), in maize pollen. Using a combination of biochemical approaches, these FAEs were partially purified and characterized with respect to their biochemical properties and putative sequences. Maize pollen FAEs were shown to be expressed early during pollen development, to release significant amounts of both monomeric and dimeric ferulates esterified from maize silks and other grass cell walls, and to synergize with an externally applied fungal endo-1,4-β-xylanase on the release of cell wall ferulates and diferulates. Preliminary analysis of maize silk cell walls following pollination, showed a significant reduction of esterified ferulates up to 96 h following pollination, compared to unpollinated silks. These results suggest that maize pollen FAEs may play an important biological role in pollen fertilization and possibly in seed production.

Indexed as

Carboxylic Ester HydrolasesCoumaric AcidsPollenPollinationSeedsZea maysCarboxylic Ester HydrolasesCoumaric AcidsBiodegradabilityFerulic acidFeruloylationMaize fertilizationPollen ferulic acid esteraseStigma cell wallZea mays

Identifiers

PMID31576447
OpenAlexW2977621031

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.