Evidence map›Paper›PMID 31576432›Full record

ArticleMetabolomics : Official journal of the Metabolomic Society2019

Metabolomic analysis of Shiga toxin 2a-induced injury in conditionally immortalized glomerular endothelial cells.

Christian Patry, Kathrin Plotnicki, Christian Betzen, Alba Perez Ortiz, Kirk L Pappan, Simon C Satchell, Peter W Mathieson, Martina Bielaszewska, Helge Karch, Burkhard Tönshoff and 1 more

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Article in Metabolomics : Official journal of the Metabolomic Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Christian PatryDepartment of Pediatrics I, University Children's Hospital Heidelberg, 69120, Heidelberg, Germany.ORCID 0000-0002-1270-6508
Kathrin PlotnickiDepartment of Pediatrics I, University Children's Hospital Heidelberg, 69120, Heidelberg, Germany.
Christian BetzenDepartment of Pediatrics I, University Children's Hospital Heidelberg, 69120, Heidelberg, Germany.
Alba Perez OrtizDepartment of Neonatology, University Children's Hospital Mannheim, University of Heidelberg, 68167, Mannheim, Germany.
Kirk L PappanMetabolon, Inc., 617 Davis Drive, Suite 400, Durham, NC, 27713, USA.
Simon C SatchellLearning and Research Southmead Hospital Bristol, University of Bristol, Bristol, BS8 1TH, UK.
Peter W MathiesonThe Principal's Office, University of Edinburgh, Edinburgh, EH8 9YL, UK.
Martina BielaszewskaInstitute for Hygiene, University of Münster, 48149, Münster, Germany.
Helge KarchInstitute for Hygiene, University of Münster, 48149, Münster, Germany.
Burkhard TönshoffDepartment of Pediatrics I, University Children's Hospital Heidelberg, 69120, Heidelberg, Germany.
Neysan RafatDepartment of Pediatrics I, University Children's Hospital Heidelberg, 69120, Heidelberg, Germany. Neysan.Rafat@umm.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionShiga toxin 2a (Stx2a) induces hemolytic uremic syndrome (STEC HUS) by targeting glomerular endothelial cells (GEC).

objectivesWe investigated in a metabolomic analysis the response of a conditionally immortalized, stable glomerular endothelial cell line (ciGEnC) to Stx2a stimulation as a cell culture model for STEC HUS.

methodsCiGEnC were treated with tumor necrosis factor-(TNF)α, Stx2a or sequentially with TNFα and Stx2a. We performed a metabolomic high-throughput screening by lipid- or gas chromatography and subsequent mass spectrometry. Metabolite fold changes in stimulated ciGEnC compared to untreated cells were calculated.

results320 metabolites were identified and investigated. In response to TNFα + Stx2a, there was a predominant increase in intracellular free fatty acids and amino acids. Furthermore, lipid- and protein derived pro-inflammatory mediators, oxidative stress and an augmented intracellular energy turnover were increased in ciGEnC. Levels of most biochemicals related to carbohydrate metabolism remained unchanged.

conclusionStimulation of ciGEnC with TNFα + Stx2a is associated with profound metabolic changes indicative of increased inflammation, oxidative stress and energy turnover.

Indexed as

MetabolomicsCell CountCells, CulturedCell SurvivalEndothelial CellsHumansInflammationKidney GlomerulusLipopolysaccharidesMultivariate AnalysisShiga Toxin 2LipopolysaccharidesShiga Toxin 2Conditionally immortalized glomerular endothelial cellsHemolytic uremic syndromeMetabolomicsShiga toxin

Identifiers

PMID31576432

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.