ArticleScientific reports2019
Proteomic characterization of primary cultured myocytes in a fish model at different myogenesis stages.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Phenotypic plasticity in fish cell lines: conceptual links to aquatic epigenetics.Frontiers in epigenetics and epigenomics · 2026Review
- Characterization of myogenesis in European sea bass (Dicentrarchus labrax) using primary white muscle cell cultures.Scientific reports · 2025Article
- A spontaneously immortalized cell line from the muscle of red sea bream (Pagrus major).Fish physiology and biochemistry · 2025Article
- Fin Cells as a Promising Seed Cell Source for Sustainable Fish Meat Cultivation.Foods (Basel, Switzerland) · 2025Article
- In vitro protein expression profile of cultivated muscle cells from Labeo rohita.Scientific reports · 2025Article
- Differentiation and Maturation of Muscle and Fat Cells in Cultivated Seafood: Lessons from Developmental Biology.Marine biotechnology (New York, N.Y.) · 2023Review
- Cysteamine improves growth and the GH/IGF axis in gilthead sea bream (Frontiers in endocrinology · 2023Article
- Myomaker and Myomixer Characterization in Gilthead Sea Bream under Different Myogenesis Conditions.International journal of molecular sciences · 2022Article
- CaThe FEBS journal · 2022Article
- Cellular Aquaculture: Prospects and Challenges.Micromachines · 2022Review
- Using Vertebrate Stem and Progenitor Cells for Cellular Agriculture, State-of-the-Art, Challenges, and Future Perspectives.Biomolecules · 2022Review
- Bioengineering Outlook on Cultivated Meat Production.Micromachines · 2022Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myogenesis is a complex two-phase process of proliferation and differentiation, which seems to be greatly conserved in vertebrates. For the first time in fish, we identify the changes that occur in the proteome during this process in a gilthead sea bream (Sparus aurata) myocyte primary cell culture (on days 4, 8 and 12), using 2-D gel electrophoresis and LC-MS/MS. A significant increase of myogenin expression at day 8 marked the transition from proliferation to differentiation. Of the 898 spots in the proteome analysis, the 25 protein spots overexpressed on day 4 and the 15 protein spots overexpressed on day 8 indicate the end of proliferation and the beginning of differentiation, respectively. Proliferation was characterized by enrichment of proteins involved in actin cytoskeleton remodelling and in cellular metabolic processes (transcription, ubiquitination, response to stress and glucose metabolism). During differentiation, 41 proteins were overexpressed and 51 underexpressed; many of them related to biosynthetic processes (RNA and protein synthesis and folding, and pentose pathways), terminal myotube formation and muscle contraction. The main cellular processes of both phases of muscle development in fish are similar with those observed in mammals but extended in time, allowing sequential studies of myogenesis.
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